The impact of calcium channel blocking agents on peritoneal cells in simulation of adhesion formation in the abdominal cavity
Abstract
It has been studied the impact of CCBA, the derivatives of phenylalkilamin (verapamil), benzothiazepine (diltiazem), dihydropyridine (nifedipine), on the function of fibroblasts and macrophages in cell culture in simulated adhesion formation. It was found that verapamil and diltiazem reduced the excessive activity of fibroblasts and mononuclear phagocytes at the stage of adhesion formation in hemoperitoneum by regulation of mitotic activity of peritoneal fibroblasts and macrophagial monocyte transformation and restriction of excessive production of pro-inflammatory cytokines TNF-a, IL-1. Verapamil was the most effective drug. Fibroblast mitotic activity was reduced by 1.8 times; and macrophage monocyte transformation was lower by 1.3 times, restricted by the excessive production of TNF-a, IL-1 in 1.5 and 2 times, respectively, that was considerably higher than in diltiazem application. No similar effect in nifedipine prescription. The results obtained are the pathogenetic rationale for further study of verapamil as a drug for prevention of adhesion development and recurrence in the abdominal cavity in clinics.
About the Authors
S. V. Skalsky
ГБОУ ВПО «Омский государственный медицинский университет» МЗ РФ
Russian Federation
Russian Federation
T. F. Sokolova
ГБОУ ВПО «Омский государственный медицинский университет» МЗ РФ
Russian Federation
Russian Federation
D. A. Sychev
ГБОУ ДПО «Российская медицинская академия последипломного образования» МЗ РФ
Russian Federation
Russian Federation
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Review
For citations:
Skalsky S.V., Sokolova T.F., Sychev D.A. The impact of calcium channel blocking agents on peritoneal cells in simulation of adhesion formation in the abdominal cavity. Journal Biomed. 2016;(3):11-17. (In Russ.)
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