The rat work capacity in forced swimming test with load and causes it variability
Abstract
The drug administration rout, body mass and atmospheric pressure effects on the rat work capacity in forced swimming test with 10% from body mass load were investigated. The rat work capacity was tested at the 1, 8, 15 and 22-nd days of intragastral or intramuscular distilled water administration. At the 8, 15 and 22-nd experimental days the rat swimming time was increased in comparison with 1-st day test results. At the 15-th day work capacity in rat group with intragastral rout was greater than in the group with intramuscular injection. The rat swimming time had inverted correlation with body mass and relative epididymidal fat mass. Atmospheric pressure had no effect on the mean swimming time in rats groups, but rats with individual sensibility to atmospheric pressure was detected. The individual sensibility of rat to atmospheric pressure was greater in rat group with intramuscular injection than in group with intragastral administration rout.
About the Authors
M. S. Zaytseva
ФГУП «НЦ «Сигнал»
Russian Federation
Russian Federation
D. G. Ivanov
ФГУП «НЦ «Сигнал»
Russian Federation
Russian Federation
N. V. Alexandrovskaya
ФГУП «НЦ «Сигнал»
Russian Federation
Russian Federation
References
1. Беляева О.А., Толстых П.И. Постинъекционные инфильтраты и флегмоны. - Киев: 1986.
2. Жуков Д.А. Биологические основы поведения. Гуморальные механизмы. - СПб: Издательство Р. Асланова «Юридический центр Пресс», 2004. 457 с.
3. Наследов А.Д. Математические методы психологического исследования. - СПб: Речь, 2004. 392 с.
4. Силантьев А.Н. Влияние метеорологических факторов на двигательную активность крыс в тесте открытого поля // Тез. ХХХ научной конференции студентов и молодых ученых Южного федерального округа. - Краснодар: Изд-во КГУФКСТ, 2003. С. 174-175.
5. Abel E.L. Behavioral and physiological effects of different water depths in the forced swim test // Physiology & Behavior, 1994. V. 56. N. 2. P. 411-414.
6. Baar K., Wende A.R., Jones T.E., Marison M., Nolte L.А., Chen M., Kelly D.P., Holloszy J.O. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1 // The FASEB J. 2002. V. 16. P. 1879-1886.
7. Baker M.A., Horvarth S.M. Influence of water temperature on oxygen uptake by swimming rats // J. of applied physiology. 1964. V. 19. N. 6. P. 1215-1218.
8. Beaton J.R., Feleki V. Effect of diet and water temperature on exhaustion time of swimming rats // Canadian J. of physiology and pharmacology. 1967. V. 45. P. 360.
9. Benjamini Y., Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing // J. R. Statist. Soc. B. 1995. V. 57. N. 1. P. 289-300.
10. Davis S., Heal D.J., Stanford S.C. Long-lasting effects of an acute stress on the neurochemistry and function of 5-hydroxytryptaminergic neurons in the mouse brain // Psychopharmacology, 1995; 118: 267-272.
11. Dawson C.A., Horvath S.M. Swimming in small laboratory animals // Medicine and Science in Sports. 1970. V. 2. N. 2. P. 51-78.
12. Fisher J.P., Seifert T., Hartwich D., Young C.N., Secher N.H., Fadel P. Autonomic control of heart rate by metabolically sensitive skeletal muscle afferents in humans // J. physiol. 2010. V. 588. N. 7. P. 1117-1127.
13. Grim M., Rerabkova L., Carlson B.M. A test for muscle lesions and their regeneration following intramuscular drug application // Toxicologic pathology. 1988. V. 16. N. 4. P. 432-442.
14. Guerreiro L.F., Pereira A.A., Martins C.N., Wally C., Goncalves C.A.N. Swimming physical training in rats: cardiovascular adaptation to exercise training protocols at different intensities // J. of exercise physiology. 2015. V. 18. N. 1. P. 1-12.
15. Hohl R., Buscariolli R.O., Ferraresso R.L.P., Brenzikofer R., Macedo D.V. Effect of body weight variation on swimming exercise workload in rats with constant and size-adjusted loads // Scand. J. lab. anim. sci. 2011. V. 38. N.3.
16. Macefield V.G., Henderson L.A. Autonomic responses to exercise: cortical and subcortical responses during post-exercise ischaemia and muscle pain // Autonomic neuroscience: basic and clinical. 2014. V. 188. P. 10-18.
17. Meijer M.K., Spruijt B.M., Zutphen L.F.M., Baumans V. Effect of restraint and injection methods on heart rate and body temperature in mice // Laboratory animals. 2006. N. 40. P. 382-391.
18. Metzger M.M., Riccio D.C. Adaptation to exercise in the rat: lack of associative control // Psychobiology. 1996. V. 24. N. 1. P. 85-90.
19. Okva K., Tamoseviciute E., Ciziute A., Pokk P., Ruksenas O., Nevalainen T. Refinements for intragastric gavage in rats // Scand. J. lab. anim. sci. 2006. V. 33. N. 4. P. 243-252.
20. Osorio R.A.L., Silveira V.L.F., Maldjian S., Morales A., Christofani J.S., Russo A.K., Silva A.C., Picarro I.C. Swimming of pregnant rats at different water temperatures // Comparative biochemistry and physiology. 2003. N. 135. Part A. P. 605-611.
21. Porsolt R.D., Anton G., Blavet N., Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments // Eur. J. of pharmacology. 1978. V. 47. P. 379-391.
22. Rosa M.J.A., Pai V.D., Neto H.B., Nouailhetas R.A., Nouailhetas V.L.A. Effect of swimming training on the rectus abdominis muscle of rats: morphological and histochemical aspects // Int. J. Morphol. 2007. V. 25. N. 3. Р. 631-638.
23. Turner P.V., Brabb T., Pekow C., Vasbinder M.A. Administration of substances to laboratory animals: routes of administration and factors to consider // J. of the Am. Assoc. for lab. animal sci. 2011. V. 50. N. 5. P. 600-613.
24. Voltarelli F.A., Gobatto C.A., de Mello M.A.R. Determination of anaerobic threshold in rats using the lactate minimum test // Braz. J. med. biol. res. 2002. V. 35. N. 11. Р. 1389-1394.
25. Voltarelli F.A., Gobatto C.A., de Mello M.A.R. Minimum blood lactate and muscle protein of rats during swimming exercise // Biol. sport. 2008. V. 25. N. 1. P. 23-34.
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Zaytseva M.S., Ivanov D.G., Alexandrovskaya N.V. The rat work capacity in forced swimming test with load and causes it variability. Journal Biomed. 2015;1(4):30-42. (In Russ.)
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