The effects of gamma irradiation on the collagen in rat tail tendon in vitro
Abstract
The effect of gamma irradiation therapy on the collagen structure of rat tail tendon has been studied.
The decrease in the denaturation enthalpy and increase in trypsin susceptibility was related with a damage of
triple helical macromolecules (19±7%). Destabilized collagen fraction and the alteration in biopolymers-water
interaction were observed by differential scanning calorimetry. The loosing of secondary and tertiary fiber
bundles was revealed by second harmonic generation microscopy. No significant degradation of anisotropic
properties and tissue architectonics was revealed by cross-polarization optical coherent tomography technique.
The instant response of collagen matrix on ionized radiation is related to degradation of collagen at the
molecular level of organization.
The decrease in the denaturation enthalpy and increase in trypsin susceptibility was related with a damage of
triple helical macromolecules (19±7%). Destabilized collagen fraction and the alteration in biopolymers-water
interaction were observed by differential scanning calorimetry. The loosing of secondary and tertiary fiber
bundles was revealed by second harmonic generation microscopy. No significant degradation of anisotropic
properties and tissue architectonics was revealed by cross-polarization optical coherent tomography technique.
The instant response of collagen matrix on ionized radiation is related to degradation of collagen at the
molecular level of organization.
About the Authors
Н. Игнатьева¹
Московский государственный университет им. М.В. Ломоносова, Москва
Russian Federation
Russian Federation
О. Захаркина²
Институт проблем лазерных и информационных технологий РАН, Троицк
Russian Federation
Russian Federation
М. Кочуева³
Нижегородская государственная медицинская академия, Нижний Новгород
Russian Federation
Russian Federation
А. Масленникова³
Нижегородская государственная медицинская академия, Нижний Новгород
Russian Federation
Russian Federation
⁴
Институт прикладной физики РАН, Нижний Новгород
Russian Federation
Russian Federation
В. Каменский⁴
Russian Federation
В. Баграташвили²
Russian Federation
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