The survival of NGF-modified embryonic stem cell in mouse multicellular blastocyst
Abstract
We investigated the effect of the microenvironment on the growth and survival of NGF-modified embryonic stem cells (NGF-ESC) with the expression of green fluorescent protein GFP in mouse multicellular blastocyst. Our results show that after the microinjection (MI), NGF-ESC migrate and co-culture primarily in the region of inner cell mass (ICM). They produce NGF and GFP and are capable for proliferation in chimeric blastocyst. However, if the cultivation period is prolonged to 120 hours, the decrease in the intensity of GFP fluorescence strength and NGF secretion is observed. We conjecture that this occurs as a result of reprogramming of NGF-ESC under the influence of ICM leading to the suppression of NGF/GFP expression and, therefore, earlier differentiation of ESC towards the neuroectoderm. Obtained results indicate that the microenvironment and cellular interaction play a key role in transferring NGF-ESC from the state of pluripotency to the neutral differentiation which is basic during the early stages of development.
About the Authors
L. M. Mezhevikina
ФГБУН Институт биофизики клетки РАН
Russian Federation
Russian Federation
E. A. Khramtsova
ФГБУН Институт биофизики клетки РАН
Russian Federation
Russian Federation
E. E. Fesenko
ФГБУН Институт биофизики клетки РАН
Russian Federation
Russian Federation
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Review
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Mezhevikina L.M., Khramtsova E.A., Fesenko E.E. The survival of NGF-modified embryonic stem cell in mouse multicellular blastocyst. Journal Biomed. 2017;(2):33-44. (In Russ.)
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