Analysis of successes and failures in obtaining transgenic mice with integrated human Nat genes and CYP3A4, based on the literature data and the results of our own research to use them as biomodels when tested pharmacological efficacy and toxicity of drugs. The analysis of possible causes of failure in obtaining transgenic mice with increased expression of human genes Nat1, Nat2, and CYP3A4, transmitting the transgene inheritance and humanized mice were done. The importance of the role of the promoter in the creation of genetically engineered structures discusses. Its own evidence that to obtain transgenic animals by microinjection method gene constructs into the zygotes pronucleus little different variants cytomegalovirus promoter (cmv). This cmv-promoter starts to include uncontrolled transgene expression at very early stages of embryo development, which often leads to his death provides. The possibility to obtain lines of humanized mice with integrated human genes Nat1 and Nat2 and a very low level of activity of endogenous Nat2 without using mice with knockout genes, Nat1 and Nat2 for crossing with transgenic mice. The analysis of our own results and causes of failures in obtaining of transgenic animals helped to get us humanized mice with integrated Nat1 and Nat2 genes, providing specific transgenes expression and create DNA-construction for animals with CYP3A4 gene.

In this article the comparative characteristic efficacy of medicines basic therapy of coronary heart disease and metabolic drugs action in experimental myocardial infarction. The studies established pathogenetic orientation metabolic efficiency means and expediency and necessity of their treatment-and-prophylactic use in cardiovascular diseases

In the present article ways of initiation of functional conditions of the nervous system which classification is offered within three-dimensional vector model of functional states are considered. The description of these ways of initiation demands definition of steady functional states and possible transients between them. Such specification of elements of model allows to create the effective tools allowing not only to diagnose functional states, but also to modulate them by means of physical methods of impact on nervous system.

Possibility of formation of supramolecular functional cluster «alcohol dehydrogenase - lactate dehydrogenase » for various сompartment of liver cells and its participation in molecular mechanisms of realization of metabolic effect of a gepatotoxic xenobiotic is shown. Cluster LDH-ADHis responsible for education dirdir in a liver of such metabolites as ethanol and a lactate, from functioning of cluster LDH-ADHdepends backback the contents in a liver of acetaldehyde and pyruvate. Prevalence of this or that substratum plays defining role in strengthening or weakening of toxic effect of tetrachlormethane.

This article describes the main methodological issues related to modeling of post-infarct heart failure by permanent and temporary occlusion of the left coronary artery, and also the most effective morphological and functional approaches to the assessment of post-infarct myocardial changes.

Fullerene C60 possible toxicity upon morphological parameters of human neutrophiles influence was investigated. Blood neutrophiles morphological parameters by nuclear and power microscopy were estimated. It was disappeared that cell soma and nuclei diameters after fullerene C60 exposure were changed significantly.

By nuclear and absorbing spectrometry the content of calcium, magnesium, iron, copper, manganese and zinc in muscle of laboratory animals at levels of food consumption, components of 100, 120 and 140% of daily requirement is studied. Statistical regularities of their interrelation in blood are revealed.

In this experimental article was investigated the influence of antioxidants on the structure of the thyroid gland of male rats of different ages in the conditions of immobilization stress. Found that prolonged immobilization, regardless of age of the animals, influences at the thyroid gland, reducing the height of the thyroid epithelium and area of thyrocyte`s nuclei and increasing the area of follicles and follicular colloid. Under the conditions of stress antioxidants, .-tocopherol and emoxipine, correct morphometric parameters of the thyroid gland in all age groups, increasing the height of the thyroid epithelium and thyrocyte`s area, reducing the area of follicles and follicular colloid. It should be noted that the analogue of the natural antioxidant - .-tocopherol exerted a stronger endocrine effects.

Morphometric analysis of Drosophila melanogaster wings revealed various change of wing`s parameters at influence by Methotrexate, Heldanamicine and Mytomicine-C, and similar changes at influence by Etoposide and Cyclophosphane. At a used dose of 2 mkg/kg of the environment of damage like “cutting” on a wing were observed only in case of influence by the Methotrexate. It is possible to assume what exactly the Methotrexate, Mitomicine-S and Geldanamicine cause big genomic instability, judging by indirect indicators.