The aim of the study was to evaluate the suitability of the use of the expression level of hypoxia-inducible factor HIF1α to diagnose the severity of tissue hypoxia. Expression HIF1α is a typical response of the genome of the cells in the hypoxic effects, triggering multidimensional mechanisms of adaptation to hypoxia, and improve the ability of tissues to experience adverse effects. Experimental hypoxia was created by the rise of laboratory Wistar rats in the chamber at a height of 6, 8, 10, 11 and 12 km. Duration of exposure varied from 30 to 5 minutes depending on altitude. Immediately after the descent of animals climbed the biological materials (whole blood, kidney, liver, heart, brain), in which method the real-time PCR determined the expression of HIF1a. As the standardization gene used was the gene "household" TSPO. It is shown that HIF1a, and TSPO have pronounced tissue-specific. Under hypoxic conditions the expression of TSPO elevation is characterized by high variability. Dynamics of expression of HIF1a elevation shows the possibility of using this indicator to assess the severity of tissue hypoxia-independent method of modeling hypoxic exposure.

The paper presents data on transplantation of splenocytes of rats with type 1 diabetes to genetically healthy animals that have been generated syndrome, appropriate clinical, biochemical and histological manifestations of the disease. Experimentally proved the existence of the transfer immune information in the formation of type 1 diabetes in animals.

The article presents the development of conditions of standardization techniques kinesis hydrodynamic study of physical health of laboratory animals. Organization of research was built on the principle of single-factor experiment. Analyzed factors were the age of the animals, the water temperature in hydraulic canals, education and training of animals prior to tiredness, stress, starvation and hypoxia. It is shown that the optimal conditions for the implementation of this research is the use of adult female rats prior training methodology and training cycle held at a temperature of water in hydraulic canals +24ºC. Preliminary hypoxic exposure causes almost a complete breakdown of health and inability to perform kinesis hydrodynamic research. Uncompensated natural processes reducing tiredness, stress and hunger cause deterioration in physical performance.

The drug administration rout, body mass and atmospheric pressure effects on the rat work capacity in forced swimming test with 10% from body mass load were investigated. The rat work capacity was tested at the 1, 8, 15 and 22-nd days of intragastral or intramuscular distilled water administration. At the 8, 15 and 22-nd experimental days the rat swimming time was increased in comparison with 1-st day test results. At the 15-th day work capacity in rat group with intragastral rout was greater than in the group with intramuscular injection. The rat swimming time had inverted correlation with body mass and relative epididymidal fat mass. Atmospheric pressure had no effect on the mean swimming time in rats groups, but rats with individual sensibility to atmospheric pressure was detected. The individual sensibility of rat to atmospheric pressure was greater in rat group with intramuscular injection than in group with intragastral administration rout.

To study the regeneration of amputated limbs of the axolotl front limb was amputated to the elbow joint. It was revealed that xenotransplantation axolotls experimental stem cell donor mice accelerates the regeneration of the remote end.

By means of biomicroscopy, using the original method of rats small intestine mesentery microvascular monitoring in an abdominal cavity, the peculiarities of microcirculatory processes disturbances caused by high doses of isoniazid, are revealed for the first time and the possibility of it correction via administration of the phytogenous lipophilic substances is proved.

A comparative study of the ultrastructure of cells of the pancreas under experimental hypothyroidism and after correction epadol. It was shown that ultrastructural changes of the exocrine pancreatic cells occur within 100 days after thyroidectomy. Destructive changes in cells with hypothyroidism are less pronounced in the correction of HRT and Epadol.

This work presents data on hypothermic storage without freezing of epididymal sperm of C57BL/6 mice in two electrolyte-free preservative buffers based on glucose and albumin, one of them supplied with 50 mM trehalose. The adding of trehalose in preservative buffer brought down significantly percentage of spermatozoa with damaged membrane after 14 days of storage as compared to solution without trehalose (1.4±0.4% vs 8.2±1.2%; p=0.00; N=16). At the same time, there were no differences between two buffers in the ability of sperm to regain motility after storage (p=0.57; N=16) as well in the chromatin fragmentation (p=0.69; N=12). Our findings might be useful for optimization of hypothermic storage protocol for human sperm in reproductive medicine.

We investigated the electrical activity of the small intestine in rats intravenously administered 200 µg/kg of lipopolysaccharide E. coli . It has been shown that disturbances in the generation of migrating myoelectric complex were observed during the 96-hours after lipopolysaccharide administration. Enteral administration of tributyrin shortened overall recovery times migrating myoelectric complex to 48 hours.

Work is devoted to studying investigationg of some aspects of pharmacological effects of silicon-, titanium-, zinc-containing glycerohydrogels of Wistar population on white rats. It was shown, that the gels increase adaptation of testing animals at treatment of thermal burn.