For the transcranial neurostimulation it is nessasry to use the right method of placing electrodes on a scalp
surface. In this work we've studied demanded precision of placing electrodes and technologies of placing
electrodes on a scalp surface.

Endogenous polyamines (putrescine, spermidine, & spermine) plays an important role in the process
of ongoing myocardial injury in the regenerative & plastic heart failure. One of the possible mechanisms
of β-blockers induced cardioprotection in the failing heart is their modulation effect on the polyamines
metabolism. Cardioselective β1-blocker nebivolol is shown to partially normalize an essentially disturbed
myocardial level of putrescine, spermidine & spermine in adriamycin-induced regenerative & plastic heart
failure in rats.

The evidence about liver lipid dystrophy, liver cirrhosis, pancreatitis with diabetes mellitus and
demyelinization nerve diseases experimental modeling are systemized. The experimental pathologies are
induced by medicine and chemical substances application in optimal doses. Two step brain insult formation on
rat is accomplish at common carotid arteria ligation with following contralateral arteria clamping after several
time. Standardization and registration time optimization for medicine efficiency estimation are realized in
morphological and biochemical testing which presented the identity of pathological changes in animal and
human. During medicine screening the adequacy of experimental model is confirmed.

In article the possible reasons of the lowered body temperature and rather low level metabolism of hairless
rats in comparison with rats with a normal hair coat and hybrid rats are analyzed. Possibly, at the given animals
deprived of a hair coat, it is connected with necessity of decrease of heat losses that forces them to move
less and more often to wash to be warmed. The specified facts do these animals attractive as models for any
biomedical researches.

The effect of gamma irradiation therapy on the collagen structure of rat tail tendon has been studied.
The decrease in the denaturation enthalpy and increase in trypsin susceptibility was related with a damage of
triple helical macromolecules (19±7%). Destabilized collagen fraction and the alteration in biopolymers-water
interaction were observed by differential scanning calorimetry. The loosing of secondary and tertiary fiber
bundles was revealed by second harmonic generation microscopy. No significant degradation of anisotropic
properties and tissue architectonics was revealed by cross-polarization optical coherent tomography technique.
The instant response of collagen matrix on ionized radiation is related to degradation of collagen at the
molecular level of organization.

Electrochemical method for analysis of Escherichia coli strain JM109 by cyclic voltammetry and square
wave voltammetry was developed. Based on the electrochemical activity of bacterial cells a system for
determining the sensitivity of microbiological material to antibiotics was proposed. Electrochemical method
provides data on antibiotic resistance of E.coli JM109 cells for 2-5 hours.

Conducting breeding selection work in population of laboratory Svetlogorsk minipigs are described.
Comparison of the basic zootechnical indicators different populations of minipigs is resulted.

In collection fund of Scientific Center of biomedical technologies under the RAMS mutant strain C57BL/
Ks-Leprdb+/+m mice is supporting, homozygote of these mice is adequate model of pancreatic diabetes II type.
The special feature is that homozygote of both sex are infertile. Strain is supporting by heterozygote crossing.
Mutant recessive gene misty promotes heterozygote selection. This gene in homozygote status refines mice
hair color. As the result of crossingover marker gene was lost, and selection of heterozygote by external
indication became impossible. Renewal of marker gene misty in genome of C57BL/Ks-Leprdb+/+m mice strain
is the aim of this work. Gene misty was transfer from strain MLR/Y in genome C57BL/Ks-Leprdb+ strain by
standard genetics and selection methods, so the name of strain C57BL/Ks-Leprdb+/+m was return back.

On the base of EEG spectral analysis we've studied the effects of piracetam and its combination with
melaxen in the conditions of cerebral blood flow disturbance in a rat model of global cerebral ischemia. It was
established, that preventive usage of piracetam & melaxen combination significantly reduces the degree of
circulatory ischemia adverse effects on the functional state of the rat brain. The obtained results can be used in
clinical practice for the treatment of ischemic and neurodegenerative brain diseases.

The conducted researches on chicken embryos and a conjunctiva of porpoises have shown that lecithin
biotechnological doesn't possess irritating activity and is safe in application for mucous membranes of
mammals. On Hodge and Sterner and K.K.Sidorov's classifications the substance of lecithin biotechnological
can be carried to almost nontoxical preparations LD50>5000 mg/kg.

Effects of intravenous administration of the novel antiparkinsonian drug hemantane and the combination
of hemantane with levodopa were assessed on the model of catalepsy induced by haloperidol in rats. It was
shown that catalepsy induced by haloperidol significantly antagonized by intravenous injection of hemantane
5 mg/kg. Combination of hemantane with levodopa increased the anticataleptic activity of levodopa. Akinetic
state was induced by low dose of haloperidol (0,1 mg/kg) in С57BL/6 with parkinsonian syndrome induced
by neurotoxin MPTP. It was shown that hemantane 5 mg/kg, intravenous, had pronounced antiakinetic and
anticataleptic activity on this model.

In experiments on animals have been spent studying of the specific activity, irritating action and toxicity of
gel Revesal structure. It has been established that anesthetizing activity is shown through 1-2 minutes, duration
of effect makes 45 minutes. Gel Revesal possesses the expressed antiinflammatory activity and healing action

Long-tern course of treatment with an antracycline antibiotic adriamycin induces a pronounced cardio-
toxic reaction with a development of specific manifestations of regenerative & plastic heart failure in rats.
Cardioselective β1-blocker nebivolol reveals evident cardioprotective activity in this heart failure model. It's
confirmed by a considerable reduction of cardiomyocytes cell death processes intensity (mainly apoptosis),
delayed activation of intracellular regeneration signs in the majority of heart muscle cells, structural stabi-
lization of myohematic barrier components, and reduction of intracellular and especially interstitial edema
manifestations.

Today depression is one of the most common forms of psychopathology. Important evaluation criterion
of the medicamental treatment effectiveness of this illness is electroencephalogram. In the experiments on
rats EEG rhythms distribution character during formed depressive disorder was analyzed after the usage of
antidepressant fluoxetin, a selective inhibitor of serotonin uptake, including its combination with medicinal
preparation of melatonine hormone melaxen.

Using model of focal brain ischemia in males of CD rats, neuroprotective properties of peptide COG1410,
with sequence of ApoE receptor-binding domen were investigated. Brain ischemia was performed by
intralumenal occlusion of middle cerebral artery with further reperfusion. Peptide was injected intravenously
30 minutes after reperfusion. For 35 days the coordination and balance of animals, and also locomotor
asymmetry were tested. In the serial slices of brain, the infarction volume was calculated. Animals, treated
with COG1410, showed reliable improvement of motor functions and reduction of infarction volume of the
affected hemisphere.

Cardioprotective properties of new encephalin analogs are studied and their
ability to raise stability of a myocardium before ischemic and reperphusion dam-
age is shown.

Primer for polymerase chain reaction (PCR) was received. This method allows genetic typing a gene of
green protein at transgene mice of line B10.GFP and opens possibility for search of donor cells with a label of
a gene of green protein at recipients after their transplantation, as more exact method.

Histologic studying of the received materials in experiment on laboratory animals (rabbits) by comparative
efficiency of the separate and combined application nanostructure β-Ca3(PO4)2 ceramics and a hydroxyapatite
(a mineral component), a complex homeopathic preparation Traumel C (a natural component) and rich
thrombocytes plasmas from autoblood has been spent.

Influence of hepatoprotective agents of phospholipids structure essentiale, eplir and its combinations with
amber acid on rats liver functional state, lipoperoxidation and bioenergetics in experimental paracetamol
intoxication was investigated. These agents demonstrated antioxidant action, decreased the common and
indirect bilirubine blood content, aminotransferase and alkaline phosphatase activity. Paracetamol uncoupled
the substrate oxidation with ADP phosphorylation and inhibited the respiratory activity of liver mitochondrions.
Essentiale and eplir increased the coupling of oxidation with ATP synthesis, in combination with amber acid
improved kinetic characteristics of liver mitochondrions.

Management and personnel requirements of GLP in experimental studies are described for conditions of
Scientific Center of Biomedical Technologies RAMS.

The paper analyzes the basic principles of designing treatment and prevention of phage preparations and
creating a collection of bacteriophages in the S-PC «MicroWorld»

The aim of the reseach was to study pharmacoplogical properties, top determine safety effect diapason
toxicoplogical properties of new adenine derivative 9-[2-(4-isopropylphenopxy)aethyl] adenine under
laboratory code VMA-99-82 which reveals antivirial activity in vitro. The results of the reseach of neurotopxic
profile of the combination VMA-99-82 are presented in the work; methodics of multitest watching accoding
to «S.Irvin» being used. In the course of the reseach in was determined safety level the substans VMA-99-82
refers to the class of low toxic copmbination. The diapopson of doses (from 18.7 to 300 mg/kg) of the substans
evident therapeutical effect in which accompanying side-effect are not essentialy revealedwas determined.
Therapeutical effect of the combination VMA-99-82 is mostly in the field of bechavioral reactions, which
presents interest for further deep stady of psychotropic effect the substance effect mechanism.

According to preliminary results, obtained on the models of thermal & chemical burns in rats an analyzed
ionized silver medicinal preparation possesses a regenerative & reparative activity in skin and/or underlied
tissues. The parameters of wound healing time are indicative of such properties, which are comparable or even
slightly exceed the same of methyluracil.