The adaptive changes in rats under 4 week’s everyday training (4 days/week) and test (1 days/week) physical exercise on treadmill was investigated. The training exercise was consisted of 20 minutes run with velocity 15 m/min and incline 15°. The test exercise was 20-30 minutes run until exhaustion with acceleration 0,6 m/min², initial velocity 12 m/min and incline 15°. The test exercise was corresponded to submaximal intensity aerobic capacity zone, and training exercise was related to medium or low intensity aerobic capacity zone by results of measurements glucose and lactate blood level. The adaptation to load were associated with the muscle hypertrophy, erythrocytes increasing, mean corpuscles volume, red cell width distribution and platelets decreasing, reduction heart rate and values of systolic pressure. The physical exercise did not influence on glucose tolerance and glucose blood level, containing glucose and glycogen in gastrocnemius muscle, wet weight of epididymal fat in rats. After 4 weeks physical load did not influence on left adrenal, thymus and spleen weights, blood level of cortisol, thyroxin, triiodothyronine and urea, liver protein and amino transferases activity, muscle lactate, protein and aspartate amino transferase activity. Therefore we suggested that physical exercise did not produce fatigue in rat.

Developed biotransplant for intra-articular introduction multipotent mesenchymal stromal cells (MMSC) adipose tissue (AT) and gel media to promote regeneration of hyaline cartilage tissue. Selection of cells based on the ability of MMSCs to differentiate into adipose cartilage cells (chondroblasts and chondrocytes) for induction to chondrodysplastic. Optimal carrier to deliver the cells in the defect, the consistency is similar to synovial fluid, supporting the viability, proliferative activity and chondrogenic potential of MSC inside the joint. It is demonstrated that with intra-articular introduction of rabbits biotransplant consisting of autologous MMSC AT and gel media on the basis of derivatives of cellulose is a complete regeneration of the defect with hyaline cartilaginous tissue.

We investigated the effect of the microenvironment on the growth and survival of NGF-modified embryonic stem cells (NGF-ESC) with the expression of green fluorescent protein GFP in mouse multicellular blastocyst. Our results show that after the microinjection (MI), NGF-ESC migrate and co-culture primarily in the region of inner cell mass (ICM). They produce NGF and GFP and are capable for proliferation in chimeric blastocyst. However, if the cultivation period is prolonged to 120 hours, the decrease in the intensity of GFP fluorescence strength and NGF secretion is observed. We conjecture that this occurs as a result of reprogramming of NGF-ESC under the influence of ICM leading to the suppression of NGF/GFP expression and, therefore, earlier differentiation of ESC towards the neuroectoderm. Obtained results indicate that the microenvironment and cellular interaction play a key role in transferring NGF-ESC from the state of pluripotency to the neutral differentiation which is basic during the early stages of development.

New metal-complex compound of iron derived vinylimidazole under the code of “Tetravim” with antihypoxic activity was studied in experiments on laboratory animals (white nonlinear mice and rats). It is shown that a single intraperitoneal introduction Tetravim at a dose of 50 mg/kg does not alter the performance of a haemogram, leads to a decrease in oxidative metabolism, the prevent of hypoglycemia that occur under conditions of acute hypobaric hypoxia (AGBH), improves hemodynamics and efficiency of oxygen exchange in the tissues, reduce the negative impact AGBH on parameters of microcirculation.

UV spectrophotometry studies have shown that various pharmaceutical drugs of Echinacea purpurea (Estifan, Echinacea-VILAR, and its purified crude drug (pharmaceutical form) possess similar spectral characteristics, typical of hydroxy-cinnamic acids, which according to the literature, determine their immunotropic activity. There have been shown that these drugs possess similar biological properties as well as safety indexes during preclinical studies. The medicines obtained from one species of plant raw materials, with similar composition of the main groups of biologically active substances, the same pharmacological properties, method of preparation, dosage form and indications, can be considered as analogues to the composition and action that allows to optimize the preclinical evaluation of their safety and efficacy.

Due to the presence of a stable tendency to progression of resistance of most significant pathogens to antifungal drugs, and also the problems of import substitution, development of new locally produced antifungal drugs is a priority direction of pharmacy and medicine. Antimycotic activity of 5 perspective compounds of silver salts pyrrolopyrazole containing sulfa fragment, against 40 clinical strains of C. аlbicans was investigated. Two most active compounds interesting for further study and identification of pharmacophore groups were identified