Application of Biomolecular Methods for Estimating Cytokine and Sirtuin Expression in Biosimulation of Ultimate Physical Load of Athletes
https://doi.org/10.33647/2074-5982-17-3-10-16
Abstract
Mini-pigs are an adequate biomodel for characterizing the processes of working capacity and endurance, as well as for conducting investigations in the field of sports medicine. For this kind of research, we propose gene targets from the families of cytokine and sirtuin proteins: IL-6, HMGB1, TNF (genes responsible for the synthesis of proteins belonging to the cytokine group) and SIRT (transferase from the family of sirtuin proteins). The SIRT 1 gene presents particular interest as an activator of mitochondrial activity during exercise. Real-time PCR systems were created, allowing assessment of the effect of various drugs on laboratory mini-pigs in preclinical studies.
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladislav N. Karkischenko, Dr. Sci. (Med.), Prof.
143442, Russian Federation, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
N. V. Petrova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nataliya V. Petrova
143442, Russian Federation, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
V. V. Slobodenyuk
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladimir V. Slobodenyuk, Cand. Sci. (Biol.)
143442, Russian Federation, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
N. A. Laryushina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nadezhda A. Laryushina
143442, Russian Federation, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
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Review
For citations:
Karkischenko V.N., Petrova N.V., Slobodenyuk V.V., Laryushina N.A. Application of Biomolecular Methods for Estimating Cytokine and Sirtuin Expression in Biosimulation of Ultimate Physical Load of Athletes. Journal Biomed. 2021;17(3):10-16. (In Russ.) https://doi.org/10.33647/2074-5982-17-3-10-16
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