Analysis of Main World Trends in Objectivization of Protocols for Behavioral Testing of Laboratory Animals with Brain Pathology
https://doi.org/10.33647/2074-5982-19-1-34-46
Abstract
Behavioral phenotyping of rodents using neurodegeneration models has received much research attention over the past three decades. However, some difficulties still exist in understanding the variability of behavior caused by genetic, environmental, and biological factors, human intervention and poorly standardized experimental protocols, which can negatively affect the interpretation of the results obtained. In this article, we discuss factors that have a negative impact on the performance of behavioral testing of laboratory animals, modern approaches to overcome them, as well as new technologies, such as visualization of neuronal activity using ion-dependent fluorescent indicators (optogenetics), which expand the boundaries of the study of neuronal networks responsible for behavior by evaluating neuronal function at both the cellular and population levels. Ultimately, this will increase the reliability of the results obtained and provide an opportunity to take a fresh look at the ethological paradigms of a particular transgenic mouse model.
Supporting agencies: this work was supported by a grant from the Russian Science Foundation (RSF) (project No. 20- 65-46004).
About the Authors
A. B. Salmina
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Health Care of Russia;
Brain Institute, Research Center of Neurology
Russian Federation
Russian Federation
Alla B. Salmina, Dr. Sci. (Med.), Prof.
660022, Krasnoyarsk, Partizana Zheleznyaka Str., 1; 105064, Moscow, Vorontsovo Pole Str., 14
Ya. V. Gorina
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Yana V. Gorina*, Cand. Sci. (Pharm.), Assoc. Prof.
660022, Krasnoyarsk, Partizana Zheleznyaka Str., 1
A. V. Bolshakova
St. Petersburg Polytechnic University of Peter the Great
Russian Federation
Russian Federation
Anastasia V. Bolshakova, Cand. Sci. (Biol.)
194021, St. Petersburg, Khlopina Str., 11
O. L. Vlasova
St. Petersburg Polytechnic University of Peter the Great
Russian Federation
Russian Federation
Olga L. Vlasova, Dr. Sci. (Phys.-Math.), Assoc. Prof.
194021, St. Petersburg, Khlopina Str., 11
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For citations:
Salmina A.B., Gorina Ya.V., Bolshakova A.V., Vlasova O.L. Analysis of Main World Trends in Objectivization of Protocols for Behavioral Testing of Laboratory Animals with Brain Pathology. Journal Biomed. 2023;19(1):34-46. (In Russ.) https://doi.org/10.33647/2074-5982-19-1-34-46
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