Protein, Lipid and Carbohydrate-Energy Metabolism Indices in Rats under Acute and Chronic Hypokinetic Stress and their Combinations
https://doi.org/10.33647/2074-5982-19-2-16-26
Abstract
This article presents the research findings regarding protein, lipid, and carbohydrate-energy metabolism indices in rats under acute (AS) and chronic hypokinetic stress (HS) and their combinations. HS and its combinations with AS were found to decrease protein metabolism indices to various extents, causing a negative nitrogen balance in the body. HS and its combinations with AS increased lipid metabolism indices, which are the factors of atherogenesis in the body (levels of total cholesterol, triglycerides, low density lipoproteins). HS and its combinations with AS increased the glucose level and decreased the creatinine kinase activity, with the values being statistically significant. AS and HS and their combinations increased significantly the lactate dehydrogenase activity.
Keywords
protein metabolism,
lipid metabolism,
carbohydrate-energy metabolism,
acute stress,
chronic hypokinetic stress,
cross-adaptation
Supporting agencies: The work was carried out within the framework of the RSF project No. 23-24-00332 “Tissue microhemodynamics: mechanisms of the anti-stress action of low-intensity millimeter radiation”.
About the Authors
M. Y. Ravaeva
V.I. Vernadsky Crimean Federal University
Russian Federation
Russian Federation
Marina Y. Ravaeva - Cand. Sci. (Biol.), Assoc. Prof., V.I. Vernadsky Crimean Federal University.
295007, Republic of Crimea, Simferopol, Akademika Vernadskogo Ave., 4
I. V. Cheretaev
V.I. Vernadsky Crimean Federal University
Russian Federation
Russian Federation
Igor V. Cheretaev - Cand. Sci. (Biol.), V.I. Vernadsky Crimean Federal University.
295007, Republic of Crimea, Simferopol, Akademika Vernadskogo Ave., 4
E. N. Chuyan
V.I. Vernadsky Crimean Federal University
Russian Federation
Russian Federation
Elena N. Chuyan - Dr. Sci. (Biol.), Prof., V.I. Vernadsky Crimean Federal University.
295007, Republic of Crimea, Simferopol, Akademika Vernadskogo Ave., 4
P. A. Galenko-Yaroshevskii
Kuban State Medical University of the Ministry of Health Care of Russia; Belgorod State National Research University
Russian Federation
Russian Federation
Pavel A. Galenko-Yaroshevskii - Dr. Sci. (Med.), Prof., Corresponding Member of the RAS, Kuban State Medical University of the Ministry of Health Care of Russia, Belgorod State National Research University.
350063, Krasnodar, Mitrofana Sedina Str., 4; 308015, Belgorod, Pobedy Str., 85
References
1. Abramova A. Yu., Pertsov A.S., Koplik E.V., Pertsov S.S. Vliyanie eksperimental'nogo khronicheskogo stressa na sostoyanie uglevodnogo obmena u krys s raznymi kharakteristikami povedeniya [Influence of experimental chronic stress on the state of carbohydrate exchange in rats with different characteristics of behavior]. Byulleten' meditsinskoy nauki [Bulletin of Medical Science]. 2018;9(1):21-25. (In Russian).
2. Aghajanian N.A., Konovalova G.M., Ozheva R.S., Urakova T.Y. Vozdeystvie vneshnikh faktorov na formirovanie adaptatsionnykh reaktsiy organizma cheloveka [The effect of external factors on the formation of adaptive reactions in human body]. Novye tekhnologii [New Technologies}. 2010;2:142-144. (In Russian).
3. Borodin E.A. Biokhimiya i klinicheskaya laboratornaya diagnostika [Biochemistry and clinical laboratory diagnostics]. Blagoveshchensk: Amurskaya GMA Minzdrava Rossii, 2021. (In Russian).
4. Butova O.A., Masalov S.V. Adaptatsiya k fizicheskim nagruzkam: anaerobnyy metabolizm myshechnoy tkani [Adaptation to physical exercise: Anaerobic metabolism of muscular tissue]. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo. Biologiya [Vestnik of Lobachevsky University of Nizhni Novgorod. Biology]. 2011;1:123-128. (In Russian).
5. Ganeeva L.A., Skripova V.S., Kasatova L.V., Nabiullina R.M., Abramova Z.I. Otsenka nekotorykh biokhimicheskikh parametrov energeticheskogo obmena u studentov-legkoatletov posle prodolzhitel'noy nagruzki [Assessment of some biochemical parameters of energy metabolism in student-athletes after prolonged exercise]. Uchenye zapiski Kazanskogo universiteta. Seriya Estestvennye nauki [Proceedings of Kazan University. Natural Sciences Series]. 2013;155(1):40-49. (In Russian).
6. Glazachev O.S., Kryzhanovskaya S.Yu. Adap-tatsionnaya meditsina: strategiya psikhofiziolog-icheskogo prisposobleniya cheloveka k kriticheski izmenennoy okruzhayushchey srede [Adaptive medicine: Human psychophysiological adaptation strategy to a critically altered environment]. Vestnik Mezhdunarodnoy akademii nauk. Russkaya sektsiya [Herald of the International Academy of Science. Russian Section]. 2019;1:48-55. (In Russian).
7. Zapadnyuk I.P., Zapadnyuk V.I., Zahariya E.A. Laboratornye zhivotnye. Razvedenie, soderzhanie, ispol'zovanie v eksperimente [Laboratory animals. Breeding, maintenance, use in the experiment]. Kiev: Vishcha shkola Publ., 1983. (In Russian).
8. Zenko M.Yu., Rybnikova E.A. Perekrestnaya adaptatsiya: ot F.Z. Meersona do nashikh dney. Ch. 1. Adaptatsiya, perekrestnaya adaptatsiya i perekrestnaya sensibilizatsiya [Cross adaptation: From F.Z. Meerson to the modern state of the problem. Part 1. Adaptation, cross-adaptation and cross-sensitization]. Uspekhi fiziologicheskikh nauk [Achievements of physiological sciences]. 2019;50(4):3-13. (In Russian). DOI: 10.1134/S0301179819040088.
9. Klyonova N.A. Biokhimiya patologicheskikh sostoyaniy [Biochemistry of pathological conditions]. Samara: Samarskij universitet Publ., 2006. (In Russian).
10. Klimov A.N., Nikulcheva N.G. Lipidy, lipoproteidy i ateroskleroz [Lipids, lipoproteins and atherosclerosis]. Saint-Petersburg: Piter Publ., 1995. (In Russian).
11. Makarov V.N., Makarova M.N. Spravochnik. Fiziologicheskie, biokhimicheskie i biometricheskie pokazateli normy eksperimental'nykh zhivotnykh [Guide. Physiological, biochemical and biometric indicators of the norm of experimental animals]. Saint-Petersburg: LEMA Publ., 2013. (In Russian).
12. Nebylcova O.V., Klimova Zh.A., Nosenko G.A., Bojko I.V., Klishnya M.A., Chernyshova L.A., Knyazeva L.P., Malikova S.V., Danilova I.E., Abasheva S.N., Degtyarenko O.A., Prishchepa L.A. Laboratornyy spravochnik Sinevo [Synevo Laboratory Reference]. Kiev: Doktor-Media Publ., 2011. (In Russian).
13. Allain C., Poon L., Chan G., Richmond W., Fu C. Enzymatic determination of total serum cholesterol. Clin. Chem. 1974;20(4):470-475.
14. Barcham D., Trinder P. An improved color reagent for the determination of blood glucose by the oxidase sys-teme. Analyst. 1972;97(151):142-145. DOI: 10.1039/an9729700142.
15. Barr D.P., Russ E.M., Eder H.A. Protein-lipid relationships in human plasma. II. In atherosclerosis and related conditions. Am. J. Med. 1951;11(4):480-493. DOI: 10.1016/0002-9343(51)90183-0.
16. Burtis C.A., Ashwood E.R., Bruns D.E. Tietz textbook of clinical chemistry and molecular diagnostics. 5th ed. St. Louis: Elsevier, 2012.
17. Cornall A.G., Bardawill C.J., David M.M. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 1949;177(2):751-766.
18. Fossati P., Prencipe L., Berti G. Use of 3,5-di-chloro-2-hydroxybenzenesulfonic acid/4-amino-phenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clin. Chem. 1980;26(2):227-231.
19. Henry R.J., Chiamori N., Golub O.J., Berkman S. Revised spectrophotometric methods for the determination of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, and lactic acid dehydrogenase. Am. J. Clin. Path. 1960;34:381-398. DOI: 10.1093/ajcp/34.4_ts.381.
20. Nauk M., Wiebe D., Warnick G. Measurement of high-density-lipoprotein cholesterol. Handbook of lipoprotein testing. 2nd ed. Washington DC: AACC Press, 2001:221-244.
21. Pisani T., Gebski C.P., Leary E.T., Warnick G.R., Ollington J.F. Accurate direct determination of low-density lipoprotein as accurate direct determination of low-density lipoprotein assay. Arch. Pathol. Lab. Med. 1995;119(12):1127-1135.
22. Porsolt R.D., McArthur R.A., Lenegre A. Psychotropic screening procedures. In: Methods in Behavioral Pharmacology. Amsterdam: Elsevier, 1993:23-51. DOI: 10.1016/B978-0-444-81444-9.50007-9.
23. Roeschlau P., Bernt E., Gruber W.A. Enzymatische Bestimmung des Gesampt-Cholesterins in serum. Clin. Chem. Clin. Biochem. 1974;12(5):226-227.
24. Schumann G., Bonora R., Ceriotti F., Clerc-Renaud P., Ferrero C.A., Ferard G., Franck P.F., Gella F.J., Hoelzel W., J0rgensen P.J., Kanno T., Kessner A., Klauke R., Kristiansen N., Lessinger J.M., Linsinger T.P., Misaki H., Panteghini M., Pauwels J., Schimmel H.G., Vialle A., Weidemann G., Siekmann L. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 3. Reference procedures for the measurement of catalytic concentrations of lactate dehydrogenase. Clin. Chem. Lab. Med. 2002;40(6):643-648. DOI: 10.1515/CCLM.2002.111.
25. Stein W. Creatine kinase (total activity), creatine kinase isoenzymes and variants. In: Clinical laboratory diagnostics: Use and Assessment of Clinical Laboratory Results. Frankfurt: TH-Books Verlagsgesellschaft, 1998:71-80.
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For citations:
Ravaeva M.Y., Cheretaev I.V., Chuyan E.N., Galenko-Yaroshevskii P.A. Protein, Lipid and Carbohydrate-Energy Metabolism Indices in Rats under Acute and Chronic Hypokinetic Stress and their Combinations. Journal Biomed. 2023;19(2):16-26. (In Russ.) https://doi.org/10.33647/2074-5982-19-2-16-26
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