Recombinant Myostatin: Isolation and Use in Vaccines
https://doi.org/10.33647/2074-5982-19-3-17-22
Abstract
Protein myostatin is not only a potential target for therapeutic effects in pathologies of the muscular system, but also a tool for biotechnological regulation of the meat productivity of farm animals. The action of myostatin can be blocked by inducing autoantibody synthesis using recombinant myostatin (rMST) as an antigen. E. coli producer strain Bl21/pET28-MSTN was obtained with a high level of recombinant protein expression in inclusion bodies. Purified rMST was used to immunize sheep and rabbits. Using the created ELISA test system for the detection of antibodies to myostatin in the blood of sheep, it was shown that rMST is immunogenic (the working titer of sera is 1/400–1/800). As a result of vaccination of rabbits over a monthly period, the average body weight and average daily weight gain in animals of the experimental groups was observed to increase compared to the control group.
About the Authors
E. M. Koloskova
All-Russian Research Institute of Physiology, Biochemistry and Nutrition of Animals — Branch of the Federal Science Center for Animal Husbandry — All-Russian Institute of Animal Husbandry named after Academician L.K. Ernst
Russian Federation
Russian Federation
Elena M. Koloskova, Cand. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institute Village
V. A. Ezerskiy
All-Russian Research Institute of Physiology, Biochemistry and Nutrition of Animals — Branch of the Federal Science Center for Animal Husbandry — All-Russian Institute of Animal Husbandry named after Academician L.K. Ernst
Russian Federation
Russian Federation
Vadim A. Ezerskiy
249013, Kaluga Region, Borovsk, Institute Village
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Review
For citations:
Koloskova E.M., Ezerskiy V.A. Recombinant Myostatin: Isolation and Use in Vaccines. Journal Biomed. 2023;19(3):17-22. (In Russ.) https://doi.org/10.33647/2074-5982-19-3-17-22
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