Production Biotechnology for a Brucellosis Antigenic Latex Diagnosticum Based on Polyacrolein Microspheres
https://doi.org/10.33647/2074-5982-20-4-18-26
Abstract
This research was aimed at developing a biotechnology for obtaining a latex brucellosis antigenic diagnosticum based on polyacrolein microspheres. This diagnosticum is intended for detecting antibodies against brucellosis in a latex agglutination reaction in human blood sera. Optimal conditions for latex sensitization with the antigen in the ratio of 1:3 at 45°C for 3 h followed by blocking of active centers with 0.5% gelatin were determined. The developed preparation showed high efficiency, which confirmed its prospects for brucellosis diagnostics. The analytical sensitivity of the diagnosticum was not less than 1:2560, in the absence of cross-reactions with heterologous sera. The diagnostic characteristics of experimental series of the preparation in control testing showed a sensitivity of 80% and a specificity of 93.33%.
About the Authors
M. M. Kurnoskina
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Mariya M. Kurnoskina
355035, Stavropol, Sovetskaya Str., 13–15
I. V. Zharnikova
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Irina V. Zharnikova, Dr. Sci. (Biol.)
355035, Stavropol, Sovetskaya Str., 13–15
D. V. Rusanova
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Diana V. Rusanova, Cand. Sci. (Med.)
355035, Stavropol, Sovetskaya Str., 13–15
A. G. Koshkidko
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Aleksandra G. Koshkidko, Cand. Sci. (Biol.)
355035, Stavropol, Sovetskaya Str., 13–15
E. V. Zhdanova
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Elena V. Zhdanova, Cand. Sci. (Biol.)
355035, Stavropol, Sovetskaya Str., 13–15
D. G. Ponomarenko
Stavropol Research Anti-Plague Institute of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing of Russia
Russian Federation
Russian Federation
Dmitriy G. Ponomarenko, Cand. Sci. (Biol.)
355035, Stavropol, Sovetskaya Str., 13–15
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Review
For citations:
Kurnoskina M.M., Zharnikova I.V., Rusanova D.V., Koshkidko A.G., Zhdanova E.V., Ponomarenko D.G. Production Biotechnology for a Brucellosis Antigenic Latex Diagnosticum Based on Polyacrolein Microspheres. Journal Biomed. 2024;20(4):18-26. (In Russ.) https://doi.org/10.33647/2074-5982-20-4-18-26
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