Effects of different concentrations of bacterial melanin on the electrical activity of neurons in the cerebral cortex in response to stimulation of the rat hindlimb nerves
Abstract
In order to detect changes in the post-stimulus electrical activity of neurons in sensorimotor cortex (SMC) induced by the influence of different concentrations of bacterial melanin, high-frequency tetanic activation of cortical neurons was studied in response to stimulation of peripheral nerves of rat hindlimb. The corresponding post-tetanic short (STP) and/or long-term (LTP) changes in the activity of cortical neurons, caused by the mobilization of a mediator, were registered. The nature of the impact was directly dependent on the concentration of the tested solution. The method of intramuscular injection is recognized as the best method for this research. The pattern of posttetanic responses was almost identical after direct application and intramuscular and/or intraperitoneal injection of melanin at use of the same concentration. The increase of neuronal activity was noted by introduction of low concentration of solution of melanin. The most reliable concentration is the concentration of 4.5 mg/ml, which induces stable and long-lasting increase in the activity of the cortical neurons. Activating effect of bacterial melanin can contribute to the recovery processes in neurodegenerative diseases.
About the Authors
T. R. Petrosyan
Ереванский государственный медицинский университет
Russian Federation
Russian Federation
V. A. Chavushyan
Институт физиологии НАН РА
Russian Federation
Russian Federation
A. S. Ter-Markosyan
НПЦ «Армбиотехнология» ГНКО НАН РА
Russian Federation
Russian Federation
A. S. Hovsepian
НПЦ «Армбиотехнология» ГНКО НАН РА
Russian Federation
Russian Federation
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Review
For citations:
Petrosyan T.R., Chavushyan V.A., Ter-Markosyan A.S., Hovsepian A.S. Effects of different concentrations of bacterial melanin on the electrical activity of neurons in the cerebral cortex in response to stimulation of the rat hindlimb nerves. Journal Biomed. 2015;1(3):52-61. (In Russ.)
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