Effect of trehalose on the safety membrane, motility and epididymal sperm chromatin fragmentation mice during hypothermic storage in electrolyte-free buffer
Abstract
This work presents data on hypothermic storage without freezing of epididymal sperm of C57BL/6 mice in two electrolyte-free preservative buffers based on glucose and albumin, one of them supplied with 50 mM trehalose. The adding of trehalose in preservative buffer brought down significantly percentage of spermatozoa with damaged membrane after 14 days of storage as compared to solution without trehalose (1.4±0.4% vs 8.2±1.2%; p=0.00; N=16). At the same time, there were no differences between two buffers in the ability of sperm to regain motility after storage (p=0.57; N=16) as well in the chromatin fragmentation (p=0.69; N=12). Our findings might be useful for optimization of hypothermic storage protocol for human sperm in reproductive medicine.
About the Authors
D. A. Isaev
ФГБНУ «Всероссийский научно-исследовательский институт ирригационного рыбоводства»
Russian Federation
Russian Federation
M. Yu. Martynova
ФГБНУ «Всероссийский научно-исследовательский институт ирригационного рыбоводства»
Russian Federation
Russian Federation
I. V. Volodyaev
Московский государственный университет им. М.В. Ломоносова
Russian Federation
Russian Federation
A. O. Revyakin
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
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Review
For citations:
Isaev D.A., Martynova M.Yu., Volodyaev I.V., Revyakin A.O. Effect of trehalose on the safety membrane, motility and epididymal sperm chromatin fragmentation mice during hypothermic storage in electrolyte-free buffer. Journal Biomed. 2015;1(4):69-76. (In Russ.)
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