Behavioral phenotypic analysis of animals with a genetic model of Alzheimer's disease

The main symptom of Alzheimer's disease is rapidly progressive dementia, which correlates with the formation of amyloid plaques and neurofibrillary proteins in tissue of the brain. This leads to the death of neurons, synoptic and cognitive dysfunction. The aim of the study was to study the development of social and cognitive impairments in the process of ontogenesis in animals with a genetic model of Alzheimer's disease. The object of the study is a genetic model of Alzheimer's disease is the B6SLJ-Tg line mice (APPSwFlLon, PSEN1 * M146L * L286V) 6799Vas, males aged 4 months. Control group - C57BL / 6 x SJL mice, males aged 4 months. Neurobehavioral testing included the following protocols: test «open field», elevated plus maze, light/dark transition test, a three-chamber social test, social recognition memory test in rodents, test «Fear conditioning». We identified features of behavioral in animals Tg 6799 line with the development of Alzheimer's disease. This increase the level of anxiety and lethargy, impaired social contacts and interactions, as well as spatial, social and associative learning and memory. Together, this indicates a disorder of emotional behavior, as well as long-term, social, spatial and emotional memory.

genetic animal model, Alzheimer's disease, memory, behavior

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18. Cubinková V., Valachová B., Brezovaková V., Szabó R., Zimová I., Kostecká Z., Jadhav S. Next generation tau models in Alzheimer's disease research - virus based gene delivery systems // Acta Virol. 2017. No. 61(1). doi: 10.4149/av. 2017. 01. 13. [Epub ahead of print].

19. Guillozet A.L., Weintraub S., Mash D.C., Mesulam M.M. Neurofibrillary tangles, amyloid, and memory in aging and mild cognitive impairment // Arch Neurol. 2003. No. 60(5). P. 729-736. doi:10.1001/archneur.60.5.729.

20. Jacobs S.A., Huang F., Tsien J.Z., Wei W. Social Recognition Memory Test in Rodents // Neuroscience. 2016. No. 6(9). doi: https://doi.org/10.21769/bioprotoc.1804.

21. Leal S.L., Landau S.M., Bell R.K., Jagust W.J. Hippocampal activation is associated with longitudinal amyloid accumulation and cognitive decline // Elife. 2017. 6. pii: e22978. doi: 10.7554/eLife.22978. [Epub ahead of print].

22. Leo L.M., Pamplona F. A. Elevated Plus Maze Test to Assess Anxiety-like Behavior in the Mouse // Neuroscience. 2014. No 4(16). doi: https://doi.org/10.21769/bioprotoc.1211.

23. Lugo J.N., Smith G.D., Holley A.J. Trace fear conditioning in mice // J. Vis. Exp. 2014. No. 85. doi: 10.3791/51180.

24. Mak E., Gabel S., Su L., Williams G.B., Arnold R., Passamonti L., Vazquez Rodríguez P., Surendranathan A., Bevan-Jones W.R., Rowe J.B., O'Brien J.T. Multi-modal MRI investigation of volumetric and microstructural changes in the hippocampus and its subfields in mild cognitive impairment, Alzheimer's disease, and dementia with Lewy bodies // Int. Psychogeriatr. 2017. P. 1-11. doi: 10.1017/s1041610216002143. [Epub ahead of print].

25. Martin L, Iceberg E. Quantifying Social Motivation in Mice Using Operant Conditioning // J. Vis. Exp. 2015. No. 102. e53009. doi: 10.3791/53009.

26. Morland R.H., Novejarque A., Spicer C., Pheby T., Rice A.S. Enhanced c-Fosexpression in the central amygdala correlates with increased thigmotaxis inrats with peripheral nerve injury // Eur. J. Pain (Lond. Engl.). 2016. No. 20(7). P.1140-1154. doi: 10.1002/ejp.839.

27. Nesteruk M., Nesteruk T., Styczyńska M., Mandecka M., Barczak A., Barcikowska M. Combined use of biochemical and volumetric biomarkers to assess the risk of conversion of mild cognitive impairment to Alzheimer's disease // Folia Neuropathol. 2016. No. 54(4). P. 369-374. doi: 10.5114/fn.2016.64815.

28. Prieto G.A., Trieu B.H., Dang C.T., Bilousova T., Gylys K.H., Berchtold N.C., Lynch G., Cotman C.W. Pharmacological Rescue of Long-Term Potentiation in Alzheimer Diseased Synapses // J. Neurosci. 2017. No. 37(5). P. 1197-1212. doi: 10.1523/jneurosci.2774-16.2016.

29. Robinson M., Lee B.Y., Hane F.T. Recent Progress in Alzheimer's Disease Research, Part 2: Genetics and Epidemiology // J. Alzheimers Dis. 2017. doi: 10.3233/jad-161149. [Epub ahead of print].

30. Seibenhener M.L., Wooten M.C. Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice // J. Vis. Exp. 2015. No. 96. e52434. doi:10.3791/52434.

31. Takao K., Miyakawa T. Light/dark Transition Test for Mice // J. Vis. Exp. 2006. No. 1. e104. doi:10.3791/104.

32. Thangavel R., Van Hoesen G.W., Zaheer A. Posterior parahippocampal gyrus pathology in Alzheimer’s disease // Neuroscience. 2008. No. 154(2). P. 667-676. doi:10.1016/j.neuroscience.2008.03.077.