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Measurement of the isotopic label incorporation in the breath co2

Abstract

This study reports a simple14C-breath test in rats. The expired carbon dioxide in the chamber was collected in a breath-sampling system using a pump after intravenous injection of14C-glucose. The level of14CO2 in the expired air was measured at 10-min intervals for 30 min. The received data confirm that this method is suitable for medical and biological investigations using labeled compounds.

About the Authors

P. P. Zolin
ФГБОУ ВО «Омский государственный медицинский университет»
Russian Federation


V. D. Conway
ФГБОУ ВО «Омский государственный медицинский университет»
Russian Federation


References

1. Денисенко Т.Л., Перфилова К.М., Неумоина Н.В., Шутова И.В., Неумоина М.В., Ефимова Е.И. Диагностика СИБР у больных хеликобактер-ассоциированной гастродуоденальной патологией // Вестник ННГУ. 2012. № 2-3. С. 41-44.

2. Западнюк И.П., Западнюк В.И., Захария Е.А., Западнюк Б.В. Лабораторные животные. Разведение, содержание, использование в эксперименте. Киев: Вища школа. 1983. 383 с.

3. Золин П.П., Лебедев В.М., Конвай В.Д. Математическое моделирование биохимических процессов с применением регрессионного анализа: монография. - Омск: Изд-во Омского гос. ун-та. 2009. 344 с.

4. Копылов Ф.Ю., Сыркин А.Л., Чомахидзе П.Ш., Быкова А.А., Шалтаева Ю.Р., Беляков В.В., Першенков В.С., Самотаев Н.Н, Головин А.В., Васильев В.К., Малкин Е.К., Громов Е.А., Иванов И.А., Липатов Д.Ю., Яковлев Д.Ю. Перспективы диагностики различных заболеваний по составу выдыхаемого воздуха // Клиническая медицина. 2013. № 10. С. 16-21.

5. Немцов Л.М. Синдром избыточного бактериального роста: актуальные вопросы диагностики и менеджмента // Вестник ВГМУ. 2015. Т.14. № 2. С. 5-15.

6. Сергиенко В.И., Бондарева И.Б. Математическая статистика в клинических исследованиях. М.: ГЭОТАР-Медиа. 2006. 304 с.

7. Степанов Е.В. Методы высокочувствительного газового анализа молекул-биомаркеров в исследованиях выдыхаемого воздуха // Труды ИОФАН. 2005. Т. 61. С. 5-47.

8. Щербак И.Г. Биологическая химия. - СПб: Изд-во СПбГМУ. 2005. 480 с.

9. Cao W., Duan Y. Breath analysis: potential for clinical diagnosis and exposure assessment // Clin. Chem. 2006. V. 52. P. 800-811.

10. Eisenmann A., Amann A., Said M., Datta B., Ledochowski M. Implementation and interpretation of hydrogen breath tests // J. Breath Res. 2008. V. 2. N 4. 046002 (9 pp) doi:10.1088/1752-7155/2/4/046002. Epub 2008 Jul 24. http://iopscience.iop.org/ article/10.1088/1752-7155/2/4/046002/pdf.

11. Gahl M.J. Benevenga N.J., Crenshaw T.D. Rates of lysine catabolism are inversely related to rates of protein synthesis when measured concurrently in adult female rats induced to grow at different rates // J. Nutr. 1998. V. 128. N 9. P. 1503-1511.

12. Kivits G.A.A., Ganguli-Swarttouw M.A.C.R., Christ E.J. The composition of alkanes in exhaled air of rats as a result of lipid peroxidation in vivo. Effects of dietary fatty acids, vitamin E and selenium // Biochim. Biophys. Acta. 1981. V. 665. N 3. P. 559-570.

13. Kneepkens C.M., Lepage G., Roy C.C. The potential of the hydrocarbon breath test as a measure of lipid peroxidation // Free Radic. Biol. Med. 1994. V. 17. N 2. P. 127-160.

14. Ledochowski M. Hydrogen breath tests. Innsbruck: Verlag Ledochowski, 2008. - 64 p.

15. Miekisch W., Schubert J. K., Noeldge-Schomburg G.F. Diagnostic potential of breath analysis - focus on volatile organic compounds // Clin. Chim. Acta. 2004. V. 347. P. 25-39.

16. Moseley L., Jentjens R.L.P.G., Waring R.H., Harris R.M., Harding L.K., Jeukendrup A.E. Measurement of exogenous carbohydrate oxidation: a comparison of [U-14C]glucose and [U-13C]glucose tracers // Am. J. Physiol. Endocrinol. Metab. 2005. V. 288. N 2. P. E206-E211.

17. Ogawa A, Murayama H, Hayamizu K, Kobayashi Y, Kuwahata M, Kido Y. A Simple evaluation method for the quality of dietary protein in rats using an indicator amino acid oxidation technique // J. Nutr. Sci. Vitaminol. 2015. V. 61. N 2. P. 123-130.

18. Phillips M., Herrera J., Krishnan S., Zain M., Greenberg J., Cataneo R.N. Variation in volatile organic compounds in the breath of normal humans // J. Chromatogr. B Biomed. Sci. Appl. 1999. V. 729. N 1-2. P. 75-88.

19. Quera R.P., Quigley E.M, Madrid A.M. Sobrecrecimiento bacteriano intestinal // Rev. Med. Chil. 2005. V. 133. P. 1361-1370.

20. Raj T., Kuriyan R., Kurpad A.V. Bicarbonate kinetics in Indian males // J. Biosci. 2006. V. 31. P. 273-280.

21. Uchida M., Endo N., Shimizu K. Simple and noninvasive breath test using 13C-acetic acid to evaluate gastric emptying in conscious rats and its validation by metoclopramide // J. Pharmacol. Sci. 2005. V. 98. N 4. P. 388-395.

22. Zolin P.P., Conway V.D. Disturbances of hypoxanthine metabolism in the liver of resuscitated rats // Bul. Exper. Biol. 1997. V. 124. N 6. P. 1180-1182. References

23. Denisenko T.L., Perfilova K.M., Neumoina N.V., SHutova I.V., Neumoina M.V., Efimova E.I. Diagnostika SIBR u bol'nyh helikobakter-associirovannoj gastroduodenal'noj patologiej // Vestnik NNGU. 2012. № 2-3. S. 41-44.

24. Zapadnyuk I.P., Zapadnyuk V.I., Zahariya E.A., Zapadnyuk B.V. Laboratornye zhivotnye. Razvedenie, soderzhanie, ispol'zovanie v ehksperimente. Kiev: Vishcha shkola. 1983. 383 s.

25. Zolin P.P., Lebedev V.M., Konvaj V.D. Matematicheskoe modelirovanie biohimicheskih processov s primeneniem regressionnogo analiza: monografiya. - Omsk: Izd-vo Omskogo gos. un-ta. 2009. 344 s.

26. Kopylov F.YU., Syrkin A.L., CHomahidze P.SH., Bykova A.A., SHaltaeva YU.R., Belyakov V.V., Pershenkov V.S., Samotaev N.N, Golovin A.V., Vasil'ev V.K., Malkin E.K., Gromov E.A., Ivanov I.A., Lipatov D.YU., YAkovlev D.YU. Perspektivy diagnostiki razlichnyh zabolevanij po sostavu vydyhaemogo vozduha // Klinicheskaya medicina. 2013. № 10. S. 16-21.

27. Nemcov L.M. Sindrom izbytochnogo bakterial'nogo rosta: aktual'nye voprosy diagnostiki i menedzhmenta // Vestnik VGMU. 2015. T.14. № 2. S. 5-15.

28. Sergienko V.I., Bondareva I.B. Matematicheskaya statistika v klinicheskih issledovaniyah. M.: GEHOTAR-Media. 2006. 304 s.

29. Stepanov E.V. Metody vysokochuvstvitel'nogo gazovogo analiza molekul-biomarkerov v issledovaniyah vydyhaemogo vozduha // Trudy IOFAN. 2005. T. 61. S. 5-47.

30. SHCHerbak I.G. Biologicheskaya himiya. - SPb: Izd-vo SPbGMU. 2005. 480 s.

31. Cao W., Duan Y. Breath analysis: potential for clinical diagnosis and exposure assessment // Clin. Chem. 2006. V. 52. P. 800-811.

32. Eisenmann A., Amann A., Said M., Datta B., Ledochowski M. Implementation and interpretation of hydrogen breath tests // J. Breath Res. 2008. V. 2. N 4. 046002 (9 pp) doi:10.1088/1752-7155/2/4/046002. Epub 2008 Jul 24. http://iopscience.iop.org/ article/10.1088/1752-7155/2/4/046002/pdf.

33. Gahl M.J. Benevenga N.J., Crenshaw T.D. Rates of lysine catabolism are inversely relat-ed to rates of protein synthesis when measured concurrently in adult female rats induced to grow at different rates // J. Nutr. 1998. V. 128. N 9. P. 1503-1511.

34. Kivits G.A.A., Ganguli-Swarttouw M.A.C.R., Christ E.J. The composition of alkanes in exhaled air of rats as a result of lipid peroxidation in vivo. Effects of dietary fatty acids, vitamin E and selenium // Biochim. Biophys. Acta. 1981. V. 665. N 3. P. 559-570.

35. Kneepkens C.M., Lepage G., Roy C.C. The potential of the hydrocarbon breath test as a measure of lipid peroxidation // Free Radic. Biol. Med. 1994. V. 17. N 2. P. 127-160.

36. Ledochowski M. Hydrogen breath tests. Innsbruck: Verlag Ledochowski, 2008. - 64 p.

37. Miekisch W., Schubert J. K., Noeldge-Schomburg G.F. Diagnostic potential of breath analysis - focus on volatile organic compounds // Clin. Chim. Acta. 2004. V. 347. P. 25-39.

38. Moseley L., Jentjens R.L.P.G., Waring R.H., Harris R.M., Harding L.K., Jeukendrup A.E. Measurement of exogenous carbohydrate oxidation: a comparison of [U-14C]glucose and [U-13C]glucose tracers // Am. J. Physiol. Endocrinol. Metab. 2005. V. 288. N 2. P. E206-E211.

39. Ogawa A, Murayama H, Hayamizu K, Kobayashi Y, Kuwahata M, Kido Y. A Simple evaluation method for the quality of dietary protein in rats using an indicator amino acid oxidation technique // J. Nutr. Sci. Vitaminol. 2015. V. 61. N 2. P. 123-130.

40. Phillips M., Herrera J., Krishnan S., Zain M., Greenberg J., Cataneo R.N. Variation in volatile organic compounds in the breath of normal humans // J. Chromatogr. B Biomed. Sci. Appl. 1999. V. 729. N 1-2. P. 75-88.

41. Quera R.P., Quigley E.M, Madrid A.M. Sobrecrecimiento bacteriano intestinal // Rev. Med. Chil. 2005. V. 133. P. 1361-1370.

42. Raj T., Kuriyan R., Kurpad A.V. Bicarbonate kinetics in Indian males // J. Biosci. 2006. V. 31. P. 273-280.

43. Uchida M., Endo N., Shimizu K. Simple and noninvasive breath test using 13C-acetic ac-id to evaluate gastric emptying in conscious rats and its validation by metoclopramide // J. Pharmacol. Sci. 2005. V. 98. N 4. P. 388-395.

44. Zolin P.P., Conway V.D. Disturbances of hypoxanthine metabolism in the liver of resuscitated rats // Bul. Exper. Biol. 1997. V. 124. N 6. P. 1180-1182.


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Zolin P.P., Conway V.D. Measurement of the isotopic label incorporation in the breath co2. Journal Biomed. 2017;(3):60-70. (In Russ.)

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