The stimulating effect of various forms of collagenic wound coverings on the epithelization process of skin wounds

Collagen represents the main structural protein of connective tissues. The chemical and biological properties of collagen determine its ability to stimulate reparative skin regeneration. Currently, a lot of wound dressings based on collagen are available for the treatment of wounds of various etiologies. However, differences in biological action of various types of wound dressings are not described in detail in the conventional in vivo models. In the present study we examined the effect of different forms of collagen wound dressings, namely, membrane, powder and hydrogel, on reparative processes in full-thickness excisional skin wounds in rats. The full-thickness skin flap in the interscapular region was surgically removed in the anesthetized animals. Wounds of rats from the experimental groups were superimposed wound dressings from collagen. The wounds in the animals of the control group were healed under the scab. On the 7th and 14th day of the experiment the animals were euthanized and the skin flaps containing wounds were excised for histological examination. The obtained histological slides were subjected to morphological and morphometric analysis. The results of the experiment revealed that the collagen wound dressings significantly accelerate epithelization of wound surface. This effect was most pronounced when applying the hydrogel as wound coverage. We hypothesize that the increased therapeutic efficacy of this form may be due to the creation of a moist wound microenvironment, as well as increased bioavailability of collagen for enzymatic hydrolysis by keratinocytes.

collagen, wound dressings, healing of full-thickness skin wounds

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