Analysis of biologically active musk compounds of musk deer (Moschus moschiferus) by gas chromatography with mass selective detector
Abstract
The study of the composition of biologically active musk components of musk deer in integrated sample preparation and subsequent determination of composition was made by GC-MS with the use of derivatization by silylation and methylation. Optimal extraction conditions, chromatographic separation and relative quantification of the main components were found. The results of identification and determination of the content of the most significant (major and minor) components in the gland tissue in accordance with the NIST library 2014 and the algorithm of relative normalization are presented in detail. Mechanisms for the transformation of macrocyclic components (muscon, exaltone), associated with the fragrant properties of the musk gland of musk deer, are proposed. Possible links between the components of the drug on the basis of the gland with a biological effect are described. According to the research results the conclusions about the multicomponent nature of the composition of the gland proposed marker ingredients in the musk gland and the possible relationship of the discovered components of the composition with the biological effects.
About the Authors
V. N. Karkischenko
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
M. S. Dulya
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
D. V. Khvostov
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
R. A. Ageldinov
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
S. L. Lyublinskiy
ФГБУН «Научный центр биомедицинских технологий ФМБА России»
Russian Federation
Russian Federation
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Review
For citations:
Karkischenko V.N., Dulya M.S., Khvostov D.V., Ageldinov R.A., Lyublinskiy S.L. Analysis of biologically active musk compounds of musk deer (Moschus moschiferus) by gas chromatography with mass selective detector. Journal Biomed. 2018;(1):19-39. (In Russ.)
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