Localization of total and Ser 129 phosphorylated α-synuclein in the rat peripheral nervous system (testing of different antibodies)
Abstract
The deposition of α-synuclein (α-Syn) in the peripheral nervous system precedes the motor symptoms of Parkinson's disease and is considered as a pathological sign and biomarker. However, data on the localization of α-Syn in the intact nervous system require clarification for the future development of experimental models and diagnostic tests. Localization of α-Syn and its the remainder of serine 129 phosphorylated form in peripheral nervous system of intact rats were studied by immunohistochemistry with use of different commercially available antibodies. α-Syn were found in presynaptic terminals of spinal dorsal horn, neuronal somata of prevertebral ganglia, nerve plexuses of vascular wall and GFAP-negative glial cells. Different antibodies to phosphorylated α-Syn shown uneven binding. Staining of processes, somata or nuclei of certain dorsal root ganglia neurons were found. No one of used antibodies detected small nociceptive IB4-positive neurons. The ambiguity of the obtained results can be related both to the different availability of epitopes detected by antibodies as a result of α-Syn interactions with other proteins, and with non-specific binding of antibodies to unidentified targets.
About the Authors
D. N. Voronkov
ФГБНУ «Научный центр неврологии»
Russian Federation
Russian Federation
O. V. Salnikova
ФГБНУ «Научный центр неврологии»
Russian Federation
Russian Federation
R. M. Khudoerkov
ФГБНУ «Научный центр неврологии»
Russian Federation
Russian Federation
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Review
For citations:
Voronkov D.N., Salnikova O.V., Khudoerkov R.M. Localization of total and Ser 129 phosphorylated α-synuclein in the rat peripheral nervous system (testing of different antibodies). Journal Biomed. 2018;(2):22-32. (In Russ.)
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