Musk deer ( Moschus moschiferus ) is a rare species of animals, the number of which is constantly decreasing. The extraction is carried out for the sake of the musk gland - "streams", therefore the object of hunting is the males. With the extraction of musk, animals are often caught in a poaching manner then they usually do not survive. The paper offers a humane method of catching musk deer for the purpose of its further enclosure keeping and lifetime organ-preserving musk production.

C57Bl/6Y mice were exposed to gamma-radiation in a sublethal dose of 7.5 Gy. In 3 hours injection 10⁶/mouse of bone marrow multipotent mesenchymal stromal cells intravenously to experimental group was done. Methods used: body weight measurement, «open field» behavior, subfraction composition of blood serum (laser correlation spectroscopy), histological examination of the spleen, liver, and pancreas, count of T- and B- cells (cytometry), white blood formula. After 1.5 and 3 months the general trend towards intermediate position of the parameters observed in the experimental between those in intact and irradiated controls attests to partial therapeutic effect of the injected cells.

Experimental data on the biological effects of drugs - derivatives of fluorene: tilorone and inhinate. Drugs act as effective inhibitors of NAT and have immunomodulatory effects about the production of IFN-gamma and TNF-alpha. Monitoring data can be used to find derivatives fluorene as promising tools in the simulation of antiviral molecules-candidates and adjuvants. Based on the data obtained in vitro experiment on enzyme activity with an effective inhibitory effect derived fluorene (tilorone and inhinate) by the enzyme N-acetyltransferase NAT2 person. For selected inhibitors studied concentration dependence of the enzymatic activity and determined the concentration value premaxillae inhibition IC50. The essence of the act fluorene derivatives to inhibit acetylation via the enzyme N-acetyltransferase (NAT1, NAT2, etc.) and through immunomodulatory effects on cytokine immune status of mice.

On the comparative analysis of the present and earlier obtained research results it have been demonstrated possibility of using the ultrasonic vocalization for differentiation animal functional states (by the example of rabbits) in this article. Recorded in activating state, generated electrostimulation with frequency 70 Hz, the ultrasonic signal was characterized by typical two-peak shape, where pikes appeared in the same frequency range that ultrasonic signal in the rest state. The activating electrostimulation made increasing of power spectral density of ultrasonic signal for each pike as compared with rest state and electrosleep state.

The method for the determination of nifedipine and dehydronifedipine in human serum for therapeutic drug monitoring in patients with arterial hypertension using HPLC-MS/MS was developed and validated. The study involved 42 patients taking nifedipine in the form of modified-release tablets. It has been shown that therapeutic drug monitoring of nifedipine is advisable to be performed both when administering dose of 30 mg (to identify patients needing to increase the dose of the drug to achieve the desired therapeutic effect), and when administering doses of 60 mg and 90 mg to identify patients with a high tendency to the development of side-effects. Simultaneous determination of nifedipine and dehydronifedipine in human serum allows to determine the phenotype of each patient according to the rate of nifedipine metabolism and to adjust the dose and the frequency of taking the drug depending on the intensity of biotransformation.

Features of adaptation of the Svetlogorsk mini-pigs of different lines and sex to an exercise stress are analyzed (running on a treadmill). The technique of training of animals to run and work with people is fulfilled and described. The percentage of easily trained and non-learning animals is revealed. Differences between these animals are described. Animals of desirable type for laying of the new specialized line are obtained.

In the work, an assessment was made of the survival and safety of the original percutaneous implant for the stump of a tubular bone made from different metal alloys. An experimental study was performed on male rabbits of the Chinchilla breed. The animals of the first group were implanted with a stainless steel product (grade EOS PH1), in the second group the implant was made of a titanium alloy (grade Ti6Al4V). Both implants are made by the additive technology of selective laser fusion by the method of layer-by-layer fusion of samples. The period of observation of the animals was 90 days after implantation. It was found that in animals of the first group two cases of implant prolapse were observed during the experiment, in the second group there were no such cases. In the first group there was one case of death of an animal, as well as three cases of chronic inflammation near the implant. With the same frequency, the animals of both groups showed an increase in the activity of transaminases and an increase in the level of the C-reactive protein. The results of the study showed that the survival rate and safety of a transdermal implant from a titanium alloy was higher relative to a product made of stainless steel.

Collagen represents the main structural protein of connective tissues. The chemical and biological properties of collagen determine its ability to stimulate reparative skin regeneration. Currently, a lot of wound dressings based on collagen are available for the treatment of wounds of various etiologies. However, differences in biological action of various types of wound dressings are not described in detail in the conventional in vivo models. In the present study we examined the effect of different forms of collagen wound dressings, namely, membrane, powder and hydrogel, on reparative processes in full-thickness excisional skin wounds in rats. The full-thickness skin flap in the interscapular region was surgically removed in the anesthetized animals. Wounds of rats from the experimental groups were superimposed wound dressings from collagen. The wounds in the animals of the control group were healed under the scab. On the 7th and 14th day of the experiment the animals were euthanized and the skin flaps containing wounds were excised for histological examination. The obtained histological slides were subjected to morphological and morphometric analysis. The results of the experiment revealed that the collagen wound dressings significantly accelerate epithelization of wound surface. This effect was most pronounced when applying the hydrogel as wound coverage. We hypothesize that the increased therapeutic efficacy of this form may be due to the creation of a moist wound microenvironment, as well as increased bioavailability of collagen for enzymatic hydrolysis by keratinocytes.