A test system for carrying out polymerase chain reaction (PCR) in real time for the purpose of verification of common or Siberian musk deer ( M. moschiferus ) was developed and proposed. This test system makes it possible to differentiate the natural musk deer’s musk from musk of other species of animals (beaver, European deer, European roe deer, moose) in a short time and with great efficiency. At the same time, the high specificity of the system is noted, which allows to control the quality of raw musk deer’s musk with a high degree of accuracy.

The study of the composition of biologically active musk components of musk deer in integrated sample preparation and subsequent determination of composition was made by GC-MS with the use of derivatization by silylation and methylation. Optimal extraction conditions, chromatographic separation and relative quantification of the main components were found. The results of identification and determination of the content of the most significant (major and minor) components in the gland tissue in accordance with the NIST library 2014 and the algorithm of relative normalization are presented in detail. Mechanisms for the transformation of macrocyclic components (muscon, exaltone), associated with the fragrant properties of the musk gland of musk deer, are proposed. Possible links between the components of the drug on the basis of the gland with a biological effect are described. According to the research results the conclusions about the multicomponent nature of the composition of the gland proposed marker ingredients in the musk gland and the possible relationship of the discovered components of the composition with the biological effects.

The paper describes the driving materials, on the basis of which it is possible to create a new effective bioactive food additive to correct erectile dysfunction. Active substances from musk of musk gland, extract of grape seeds, red root (Hedýsarum), biomaterials of spotted deer were obtained in Scientific Center for Biomedical Technologies of FMBA of Russia. Preclinical studies of their efficacy, safety, pharmacokinetics and dosing have been started. The work on the selection of the most cost-effective technology for the isolation of active components from natural raw materials for the development of experimental industrial regulations is being completed. Based on the active substances obtained, it is planned to create a new bioactive food additive to correct erectile dysfunction.

An overview of the currently available genetic biomodels of the metabolic syndrome used to study the pathophysiological foundations of the development of the disease and are suitable for the search and evaluation of new effective ways of treating this disease.

The effect of thiamazole in daily doses of 20; 10; 5; 2,5 and 1 mg / 100 g of body weight on thyroid, liver and hormone levels of the pituitary-thyroid system (free thyroxine, total 3,5,3'-triiodothyronine and thyroid-stimulating hormone) has been studied. The study has shown that high doses of thyrostatics (20; 10 and 5 mg / 100 g of body weight) induced symptoms of a multi-organ pathology of toxic origin and do not reflect the processes characteristic of hypothyroidism. In order to simulate thyroid dysfunction by the type of endemic effect, it is recommended that thiamazole at a dose of 2,5 mg / 100 g of body weight ensure a steady decrease in the mesic circadian rhythms of body temperature and the functional activity of the thyroid system against the background of micromorphological transformations in the thyroid and liver. Relatively low doses of thiamazole (1 mg / 100 g of body weight) at the same time of administration do not provide a steady decrease of body temperature in all phases of the circadian rhythm (7 am, 11 am, 15 pm, 19 pm, 23 pm).

The social isolation effect on endurance to stress and bone resorption in rat under thermal stress was investigated. The rats 2-2.5 months old were placed in social isolation for 30 days. The influence of daily thermal stress for 7 day on rats that were being in social isolation or in group was studied. The social isolation decreased of rat anxiety in open field, declined of blood and adrenal 11-oxycorticosteroids level, brain norepinephrine level and elevated adrenal epinephrine level, blood calcium level and femur density. In group being rat thermal load activated hypothalamo-pituitary-adrenal axis and sympathoadrenal system, elevated bone resorption, that were observed in increase of blood free hydroxyproline level, alkaline phosphatase activity and blood phosphorus level, reduction of blood calcium level and femur density. In rats that were underwent social isolation thermal load induced no changes of hypothalamo-pituitary-adrenal axis and bone resorption biochemical parameters, but elevated left adrenal relative mass and decreased femur density. This result suggest of positive social isolation effect on endurance to stress and bone resorption in rat under thermal stress.

Transcranial direct current stimulation (tDCS) is a modern non-invasive method of physiotherapy leading to the selective activation of the opioidergic system. This article presents data of tDCS’s influence on the endurance of rats with different vulnerability to stress. The level of vulnerability was researched by the test of a coercive swimming with freight. In the first test the division of rats into three groups of low, medium and high stress vulnerability took place. After 5 sessions of tDCS there was a reassessment of animals’ vulnerability in the test of the coercive swimming with freight. Analysis of the swimming time showed a positive effect of tDCS on the endurance of rats. An especially expressed effect was observed in groups of animals with low and high stress vulnerability.