The aim of this study was to presents a model-based approach based on the integration of computational theory (in silico-experiments) and experiments performed (in vitro) to search, optimization and development of new anticoagulants molecules with affinity to the family of serine proteases. The authors gratefully acknowledges to Novartis AG for assistance in the in vitro experiments in the framework of the program “Novartis in-kind collaborations”, as well as the personal gratitude for their researchers and biologists Ulrich Hassiepen and Gabriela Monnet.

The classification of functional status suggested in terms of the three-dimentional vector model of human functional states was described in the article. We had compared this classification with others experimental data and as a result of their relative analysis it was revealed that typical combination of diagnostic markers correspond to every functional state.

The classification of functional status suggested in terms of the three-dimentional vector model of human functional states was described in the article. We had compared this classification with others experimental data and as a result of their relative analysis it was revealed that typical combination of diagnostic markers correspond to every functional state.

In order to restore defect skeletal muscle tissue was used allogeneic spongy biomaterial (AGB). Used histological, immunohistochemical and electron microscopic methods. The use of AGB in a deep damage to the muscles of the calf rats contributed to a full restoration of the defect. The products of biodegradation of biomaterial are chemoattractant for resident macrophages (CD 68) and stimulate their phenotypic maturation that determines the outcome of regeneration. In the group of animals without the use of biomaterial in the healing process of the defect were scarce CD 68 cells and their functional inactivation. The result was the formation of a scar with a further degeneration in the adipose tissue.

Single injections of nanopeptide C56H78N16O12 in a dosage of 2 cm3 of solution concentration of 5 mkg/ml (10 mkg / an individual) steadily induce a sperm allocation at males of sturgeon fishes in the conditions of cultivation. Standard criterions of quality ejaculate and results of functional testing showed their physiological full value and suitability for use in the course of reproduction

Possibility of use the poycilotermes hydrobionts for carrying out model experiments is shown. Successful modeling of pathology of a liver and pancreas at carps, hepatopancreas crawfishes is carried out.

The present paper studies the seizure activity dynamics at different concentrations of sex steroids using electroencephalographic method. For the first time the experimental animal study shows the influence of normal level of female sex hormones with their deficit and after hormone replacement therapy with 17.-estradiol and progesterone on the quantitative and qualitative characteristics of spike-wave discharges. The animals being observed are WAG/Rij female rats representing a genetic model of generalized absence epilepsy in humans. It has been experimentally established that estrogen and progesterone are involved in the spike-wave discharges formation mechanisms accompanied with absence seizures in WAG/Rij female rats.

The method of a nuclear and absorbing spectrometry studied the content of calcium, magnesium, iron, copper, manganese and zinc in liver of laboratory animals at levels of food consumption, components of 100, 120 and 140% of daily requirement. Statistical regularities of their interrelation in liver are revealed.

Work is devoted to research of consequences of influence of acute hypobaric hypoxia on female gametes of rats and mice. In experiment the rats of the strain HY/Y low-steady against a hypoxia removed and supported in SCBMT and a mouse strain C57BL/6Y, supported also in our Center were used. Virgin females of rats and mice for 9-16 days of the post-hypoxemic period coupled to intact males to reveal consequences of influence of the general hypobaric hypoxia on female gametes. The accounting of embryonic mortality in the uterine period of development carried out, opening females for 19-20 days of pregnancy. As a result of the conducted research the following is established. At the general mortality of embryos in the post-implantation period of development in 39,1% at females of rats and - 38,73% at females of mice, the share of mortality of the embryos, caused by influence of a sharp hypoxia, made at them 32,89% and 31,76%, respectively. Losses from among ovulated ova at a preimplantation stage at females in experience made at rats 14,3%, at mice - 26,7%. Thus, under the influence of acute hypoxia on females gametes of rats females of mice had total losses equal 47,16%, - 58,45%. It should be noted also that 6 females of rats and 4 females of mice in experience had no any implant in general. While at control animals of both types it isn’t revealed any case of their absence. It is explained, apparently, by that it, was caused by impact of a hypoxia on a female in the period of her especially sensitive stage of an ovogenesis that probably finds confirmation in wide scope of a variation of size of this indicator at females of skilled groups.

The study of the basic parameters of rhythmographic research mini-pigs cardiac under normal conditions of stay in the vivarium conditions and stress-induced tachycardia exercise and its mathematical analysis, and the use of indirect anticholinergic and cholinomimetic galantamine metatsina. It is shown that the autonomic regulation of the heart during stress-induced tachycardia and pharmacologic effects not fundamentally different from similar adjustment in people that allows us to consider a mini-pigs adequate biological model to assess the effects of pharmacological agents on the autonomic nervous system during various modeling studies of short-term pathological processes.

In article the data obtained experimentally on toxicity of representatives of various groups of inhibitors of system of RAAS are generalized: renin inhibitors (aliskiren), inhibitors of angiotenzintransformation enzyme (AAE) (enalapril, fosinopril), blockers angiotensin II (ATII) of receptors (valsartan, losartan, telmisartan, irbesartan). Researches of toxicity are conducted on the outbred rats at single and repeated (30 days) intragastric introduction in the doses equivalent of 1, 10 and 20 of highest to therapeutic for persons. By results of researches all studied representatives are carried to a class of low-toxic medicines. Toxic influence is revealed after repeated introduction of preparations in doses of 10 and 20 highest therapeutic. Main target organs of toxic influence: cardiovascular system, kidneys, liver is the minimal influenced.

Rats with streptozotocin-induced diabetes have the hemorheological disturbances which appeared in increase of blood viscosity, elevation of erythrocyte aggregation and decrease of red blood cell deformability. In streptozotocin-induced diabetic rats the concentrations of total lipids, phospholipids (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine) were decreased and contemporary the content of sphingomyelin and lysophospholipids were enhanced. Administration of extract from Rhaponticum carthamoides (150 mg/kg, i.g., during 14 days) contributed to the decrease of blood viscosity, depression of erythrocyte agregation, improve of erythrocyte deformability and increase of blood’s overall oxygen transport capacity in comparison with controls. Streptozotocin-induced diabetic rats, treated with Rhaponticum carthamoides extract showed the elevation of total lipids, restored phospholipids ratio of external monolayer (phosphatidylcholine, sphingomyelin) and internal monolayer (phosphatidylethanolamine, phosphatidylserine) and also diminution of lysophospholipids in erythrocyte membranes. Extract from Rhaponticum carthamoides contributed to reduction of glucose level in blood and prevent of reduction body mass of streptozotocininduced diabetic rats.

Under hypoxic conditions of different genesis decreases the affinity of hemoglobin for oxygen by increasing the synthesis of 2,3-diphosphoglycerate. Course administration ethylmethylhydroxypyridine malate promotes restoration of activity of 2,3-DPG and the normalization of blood oxygen tension in patients with CHF for 5 days.

Objectives were to determine whether C3435T polymorphism in ABCB1 gene is associated with efficacy and safety of bisoprolol treatment in Russian pregnant women with mild to moderate chronic hypertension. The comparative analysis of allele/genotype frequencies of ABCB1 gene C3435T polymorphism was undertaken in Russian pregnant women with mild to moderate chronic hypertension (N1=65) and healthy subjects on the similar periods of gestation (N2=86). The data suggest that C3435T polymorphism in ABCB1 gene is not associated with efficacy of bisoprolol treatment. The maternal ABCB1/C3435T polymorphism showed association with birth weight and Apgar score.