The present message is devoted to search of physiological markers of a condition of an electric sleep. Transcranial electric sleep is artificially generated by using the electric current analogue of natural sleep. On healthy volunteers athletes it is shown that the condition of an electric sleep is followed by increase in values of indicators of respiratory and warm activity, more characteristic for a condition of a relaxation, than for a condition of a natural dream. Along with it introduction of a Paroxetine (a selective inhibitor of reuptake of serotonin (SIRS)) before training and procedure of an electric sleep promotes change of dynamics and improvement of indicators of course of physiological processes. Application of a Paroxetine in controlled research improves a sleep, promotes turning on of relaxation mechanisms of an electric sleep. The melatonin role as possible intermediary in processes of a relaxation is discussed.

The orphan receptors are brought the review of modern literature on endogenous ligands of large group. It is shown that intermediates of carbohydrate, fatty and protein metabolism, a cycle the three-carbonic acids actively participate in regulation of exchange processes, and their synthetic analogs - ligands to orphan receptors (both agonist, and antagonists) are perspective objects of development new drugs at a wide range of diseases (diabetes, obesity, a metabolic syndrome, autoimmune diseases, a hypertensive illness, myocardium ischemia, a myocardium hypertrophy, neurodegenerative diseases, immunodeficiency, liver diseases, etc.). Concerning several ex-orphan receptors potentially perspective chemical compounds which can act as ligands to receptors of metabolic type of activation are presented.

Whole body cryotherapy (WBC) effects researches are organized to reveal and collect new clinical results on negative and positive aspects of cold therapy. WBC is a method of body exposing to low temperatures. Nitrogen gas is cooled to -130 С, which lowers the patient's skin surface temperature. Some scientists and doctors see a potential health menace in low temperature use. They suggest starting clinical practice on laboratory animals to minimize the risk of WBC. It is advised to start experiments on rats and mice. For a comprehensive view of clinical practice, it is recommended to evaluate the cryogenic effects on laboratory animals. It is also necessary to understand what scientific and practical value these studies will have and how these findings can be applied.

The work presents the development of a methodology and optimization of recombinant N-acetyltransferase-2 of human (hNAT2), found the optimal parameters of use of the enzyme in a soluble form and in sufficient quantity for the in vitro bioanalytical experiments. The paper presents data on the design of genetic constructs for efficient expression of recombinant enzyme human N-acetyltransferase 2, by the method of SDS-PAGE electrophoresis confirmed the molecular weight of the protein (34-35 kDa), indicated quantitative content of enzyme (3.14 mg/ml).

The experiment showed successful regeneration of hepatopancreas of crawfishes after a xenotransplantation of stem cells from mice-donors.

During this work it was studied the influence of the new entretenimiento of natural origin (DPP-4 inhibitor, the drug KLS-073) on basic biochemical parameters for a long period (12 months) of application. The experiment was carried out on outbred mice of both sexes. The introduction of the drug was carried out during 12 months in three doses: higher therapeutic (ITD) (2 mg/kg), 5 ITD (10 mg/kg) and 10 ITD (20 mg/kg). As of the evaluation criteria in the assessment we used overall biochemical and hematological parameters and histological examination of the thyroid and the pancreas over time (the 31st, the 91st, 181st and 361 th days). According to the results, the study showed no differences in biochemical parameters and structure of the thyroid and pancreas glands of intact animals and animals treated with the study drug KLS-073.

In experiments on rats using a test “hot plate” and the registration of the latent period of the reaction licking feet potentiating effect of heparin on the antinociceptive FMRFa effects was established. Protamine sulphate, Naloxone and Obzidan reduced antinociceptive FMRFa effects. The heparin blocked the anti-analgetic action of Protamine sulfate, Naloxone and Obzidian.

The aim of the study was to detect single nucleotide polymorphisms (SNP) near gene IL28B on locus rs12979860 (С>T) and locus rs8099917 (T>G), which are prognostically significant for standard dual therapy in patients with chronic hepatitis C of Russian (Europian) and Tyvinian (Mongoloid) ethnicity. The SNP were discovered by real-time PCA. The Tyvinian patients more frequency showed the C-allele, and Russian patients more frequency displayed Т-allele (p=0.0466). There were no differences in T- and G-alleles on locus rs8099917. The Tyvinian patients more often showed genotype C/C (p=0.0231) and Russian patients more frequency displayed genotype C/Т (p=0.0425) on locus rs1297960. Genotype T/T on locus rs8099917 significantly often detected in Tyvinian patients (p=0.0259) and genotype T/G more frequently detected in Russian patients (p=0.0188). When take in account two polymorphic loci, genotype С/С (rs12979860) - Т/Т (rs8099917) significantly often detected in Tyvinian patients (p=0.0203), this genotype is associated with more ability to get sustained virologic response.

Data of the experimental modeling of acetaminophen-induced disturbances of the bile producing liver function in rats, maintained under the different regimen of the protein nutrition are submitted. It is shown, that alimentary deprivation of protein is the critical factor, aggravating the course of the acetaminophen-induced disturbance of the bile producing liver function. The used method of per os administration of acetaminophen in a starch suspension at dose 1 g/kg of body weight during 2 days after the 4-weeks maintenance of rats under the conditions of alimentary deprivation of protein, allows to obtain the model of the disfunction of a bile production by liver.