Clinically relevant model for the study of antihypoxic activity drugs was developed. It is shown that repeated short-term moderate hypoxic exposure (pregnant female rats on days 9-12 of gestation stay in the chamber at daily 9000 m altitude for 30 min) caused marked fetal hypoxia, accompanied by a significant increase in the higher rate of embryonic lethality to the 20-21 day of pregnancy. Antihypoxic activity drugs (Trimetazidine, Actovegin, Piracetam) introduced intragastric before 1 hour of hypoxia exposure prevent hypoxia-induced increase in embryonic lethality.

It has been studied the impact of CCBA, the derivatives of phenylalkilamin (verapamil), benzothiazepine (diltiazem), dihydropyridine (nifedipine), on the function of fibroblasts and macrophages in cell culture in simulated adhesion formation. It was found that verapamil and diltiazem reduced the excessive activity of fibroblasts and mononuclear phagocytes at the stage of adhesion formation in hemoperitoneum by regulation of mitotic activity of peritoneal fibroblasts and macrophagial monocyte transformation and restriction of excessive production of pro-inflammatory cytokines TNF-a, IL-1. Verapamil was the most effective drug. Fibroblast mitotic activity was reduced by 1.8 times; and macrophage monocyte transformation was lower by 1.3 times, restricted by the excessive production of TNF-a, IL-1 in 1.5 and 2 times, respectively, that was considerably higher than in diltiazem application. No similar effect in nifedipine prescription. The results obtained are the pathogenetic rationale for further study of verapamil as a drug for prevention of adhesion development and recurrence in the abdominal cavity in clinics.

A study of the effects of exposure to sharp hypobaric hypoxia on the reproductive cells of male and female rats and mice was held. In the experiment, low resistant to hypoxia NU/SmY lines of rats and mouse line C57BL/6Y had used, all of it was bred and maintained in SCBMT of FMBA of Russia. Sexually mature male and female rats and mice were exposed to acute hypobaric hypoxia on 9-18 day period posthypoxic mated (by type). To determine the effects of acute hypoxic stress in the germ cells of rats and mice to account embryonic mortality in the uterine period of development, the female opened by 19-20 days of pregnancy. It was found that the overall mortality in post-implantation embryos period, equal to 48.11% in female rats and 49.71% in female mice, the proportion of deaths due to the influence of acute hypoxia was 40.57% and 43.24%, respectively. Losses among the ovulated eggs in the pre-implantation stage of development in rats were 22%, in mice - 8%. Thus, the total loss caused by exposure to sharp hypobaric hypoxia exposure on male and female reproductive cells, accounted for 62.57% in female rats, in female mice - 51.24%.

The aim of this work was the establishment of a subsequent investigation of streptococcal wound infection model induced by subcutaneous administration of the bacteria strain of Streptococcus pyogenes Dick-1 by BALB/c mice and Wistar rats. As a result of experiments, it was found that the weight and organ colonization of animals infected with the culture S. pyogenes Dick-1 is one of the main indicators of the physiological state of the animal. This histological analysis provides evidence that infection with streptococcus intradermal occurs defeat the epidermis and dermis with generalizations of the pathological process in the parenchyma organs (lungs, spleen, liver, kidneys). During observations of the infectious process there was a significant reduction in contamination of the wound surface among mice by the end of the experiment on day 21 single colonies were inoculated. Analysis of the results showed that the spread of infection among mice begins with lung disease, since the 7th day we can observe generalization of infection with the penetration in other parenchyma organs. The washes from the wound surface in rats, in contrast, showed a significant streptococcal colonization. Locating the source of infection occurred in the lungs, a condition of other parenchyma organs remained within normal limits.

We have modified the model of swimming training and evaluated some physiological changes, developing during the training process. In response to aerobic training load animals have evolved applied compensatory bradycardia, increased overall endurance. The proposed training regimen (swimming twice a day for 90 min with a break of one hour (except the first three days where the workout 1 hour per day), five days a week for 4 weeks) to evaluate the effectiveness of the training process (adaptation to physical exercises) in the experimental animals and the influence of the pharmacological agents.

The action of Mexicor on lipid peroxidation (LPO) and motor function in the conditions of post-traumatic period in the simulation of traumatic brain injury (TBI) in rats was studied. It was revealed that the head injury was a significant increase in the content of lipid peroxidation products (conjugated diene, triene conjugates concentration of malondialdehyde and Schiff bases) and decreased motor function of animals. Application of Mexicor in head injury led to a decrease in the concentration of lipid peroxidation products and the restoration of motor activity compared with the control. The most pronounced changes during the action of Mexicor registered on 3-7 day of post-traumatic period. The findings revealed the efficiency of Mexicor for rats with TBI, which was reflected in the normalization of lipid peroxidation and reducing the timing of the motor dysfunction.

The article presents data on the dynamics of hematological parameters of blood of cats to control the effectiveness of treatment of purulent wounds probiotics. Probiotics-sorption preparations "Dilaksil" and "Sorbelakt" in complex treatment of cats with purulent wounds found to be effective. Their use has a positive effect on hematological parameters of blood of experimental animals, cleaning the wound from purulent exudate, reduces the time of the beginning of the epithelialization of wounds and their complete healing.

The paper shows preliminary preclinical findings of new potassium salt of 2-[3-bromine-1-(thietanil-3)-1,2,4-triazolil-5-thio]acetic acid in regards to hemostasis system. The research has determined that potassium salt of 2-[3-bromine-1-(thietanil-3)-1,2,4-triazolil-5-thio]acetic acid shows high antiaggregational activity under condition in vitro and in vivo , it is more efficient as means to experimentally prevent generalized thrombosis in comparison with the practically applied therapeutic agents. The findings allow to consider this compound a promising antithrombotic drug.