Preparation of Differentiated Recombinant Human β2-Microglobulin and Mouse β2-Microglobulin Proteins for its Detection in Class I HLA Chimeric Molecules
https://doi.org/10.33647/2074-5982-20-2-21-31
Abstract
Transgenic humanized animals are increasingly in demand for biomedical research and pharmacological testing. More and more lines of transgenic animals are being created, including those with knockout of their own genes. There is an urgent need for an evidence base for the integration of a transgene, its expression, determination of the knockout state of its own gene at the molecular genetic level, detection of translation of the target protein in different organs and tissues, proof for the absence of protein synthesis (or its non-functionality), the gene of which has been modified. This requires highly specific reagents, proteins and antibodies to them in particular, the vast majority of which are presented by foreign manufacturers. The task was set to identify mouse and human β2-microglobulin in protein fractions of organs and tissues of transgenic and knockout mice of several HLA lines created in recent years at the Scientific Center of Biomedical Technologies, Russia. At the first stage of our research, recombinant E. coli producing strains were obtained.
Supporting agencies: the research topic “Generation of a new line of humanized transgenic mice carrying the human HLA-A*pX gene and knockout of the mouse H-2K complex” (code: “Transknockout-2024”) of state assignment of Scientific Center of Biomedical Technologies of FMBA of Russia.
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladislav N. Karkischenko, Dr. Sci. (Med.), Prof.
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
V. A. Ezerskiy
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Vadim A. Ezerskiy
249013, Kaluga Region, Borovsk, Institute Village
E. M. Koloskova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Elena M. Koloskova, Cand. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institute Village
M. S. Nesterov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Maxim S. Nesterov
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
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Review
For citations:
Karkischenko V.N., Ezerskiy V.A., Koloskova E.M., Nesterov M.S. Preparation of Differentiated Recombinant Human β2-Microglobulin and Mouse β2-Microglobulin Proteins for its Detection in Class I HLA Chimeric Molecules. Journal Biomed. 2024;20(2):21-31. (In Russ.) https://doi.org/10.33647/2074-5982-20-2-21-31
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