The main method of support of high level human efficiency in extreme condition was using pharmacological psychostimulating agents in practice of emergency and disaster medicine. As a result of high level stress effect as known probability of superfluous stimulatory action had lead to nervous system hyperactivation. Possibilities of reduction nervous system hyperactivation after physical activity and mental stress by transcranial electrocerebral rhythmical stimulation methods on the background of the stimulant action or without it modeling in laboratory rabbits discussed in this article. We had been shown on basis of heart and respiratory activity indices effectiveness of diagnostics of inhibitory effect on physiological system by the low-frequency rhythmical electrical subcortical brain stimulation. Respiration rate was more sensible to changes of animal functional status then heart rate. Duration of electrocerebral stimulation should increase by 5 min minimally subject to nervous system hyperactivation as compared with normal condition.

At present time, diabetes is regarded as polygenic, multifactorial disease: genetic predisposition in combination with environmental triggers activates specific autoimmune processes, which lead to the death of β-cells. Therefore, violations in the processes of initiation and implementation of apoptosis are fundamental in the development of the disease. Preservation or restoration of pancreatic β-cells in diabetic patients is an ultimate therapy for diabetes, but is an unmet medical challenge Protein kinase MST1 has been recently identified as a key mediator of cell apoptosis, and recent studies have shown that it could serve as a target for developing new drugs to treat diabetes. In order to find active compounds capable to inhibit MST1 (Mammalian sterile 20-like kinase 1), we carried out a computer-aided design, using methods based on the structure of macromolecule-target (Target-Based Drug Design) and methods based on the structure of ligands (Ligand-Based Drug Design). To assess the undesirable effects we used the computer program PASS (Prediction of Activity Spectra for Substances); for construction of quantitative relationships «structure-activity» (Quantitative Structure-Activity Relationship) and evaluation of inhibitory activity computer program GUSAR (General Unrestricted Structure-Activity Relationships) was used. As a result of experiment, 6 potential inhibitors of MST1were selected. These compounds do not exhibit mutagenic or toxic properties but have high level of activity in comparison with already known inhibitors. This fact makes it possible to suppose, that our compounds are suitable for future synthesis and in vitro screening.

Statistical analysis of the laws that determine antihypoxic activity of the compounds, which salts energomash acids Krebs cycle was performed. The antihypoxic activity level of the studied compounds comparable with the level of Piracetam and Preductal. It was shown that the choice of anion substantially affects the efficiency of antihypoxic activity of such salts. The most active compounds were succinate and L-malate. L-malate antihypoxic activity in 1,5 times exceeds a corresponding activity of racemic salt.

The problem of competent depression diagnostics and treatment in medical practice is quite obvious and actual. In this case pharmaco-EEG represents one of the most reliable methods of pharmacotherapy efficiency assessment. We’ve analysed cerebral EEG rhythms distribution character in conditions of experimental depression in rats, including antidepressants (Valdoxan and Fluoxetine) usage. It was established that antidepressants induces animals exploration activity increase, as well as significant potentiation of normally dominating EEG theta-rhythm activity in rats. The similar EEG-rhythm distribution analysis was carried out in patients with depression, including the period of Valdoxan and Fluoxetine pharmacotherapy. It is noted that antidepressants usage significantly improves current condition, inducing normal distribution of alpha-rhythm and reduction of slow-wave activity on EEG.

The aim of the work was the study of patients with persistent hypertension functional reserves of cells and to evaluate the significance of the impact of genetic and immunological mechanisms in the functional state of the function of the endothelium. The work is based on the results of a survey of 42 patients with persistent arterial hypertension. As a result, it was found that in patients with persistent arterial hypertension in 66,7% of observations metabolic stability of endothelial cells to oxidative stress is preserved, while the 33,3% results in a loss of resistance to the action of cytotoxic products. The cytokine system when there are hypertension refractor two closely interrelated processes: the imbalance between the overproduction of inflammatory cytokines (tumor necrosis factor-α, interleukin-6) and anti-inflammatory cytokines (interleukin-4, interferon-γ) dominated the first over the second. Persistent arterial hypertension patients with absence of metabolic resistance ability of endothelial vasodilation during stimulation of chemo-and mechanoreceptors in progressively decreases, which is associated with increased levels of proinflammatory cytokines in the blood and the presence of polymorphic variants «-174 G/C» to the interleukin-6 and «308 G/A» in the tumor necrosis factor-α.

Influence of different doses of ethanol on parameters of vocalization of rats in the ultrasonic range is investigated. It is established that accumulation of ethanol is reflected in change of frequency spectral characteristics of ultrasonic vocalization of rats. The ethanol entered to rats in doses, approximately equal 1, 3 and 4 g/kg forms a positive affective state, and in doses of 2 and 5 g/kg - negative. Ultrasonic vocalization of the rats who received 3 g/kg of ethyl alcohol can reflect euphoria signs, and close to lethal the dose of 5 g/kg causes detection in ultrasound of signs of a distress. The model of research of a psychoemotional state on vocalization of rats in ultrasound can be recommended for preclinical tests of remedies for alcoholic dependence.

According to the International Documentation requirements of the parameters and the criteria of the biological activity estimation and specificity of Purified Allergen of atypical mycobacterium weve found out. The expediency of definition of size biological activity of Purified Allergen of atypical mycobacterium on the guinea pigs infected with mixture of mycobacteria M. intracellularae and M. scrofulaceum is shown. It is shown, that the definition of a parameter of Purified Allergen of atypical mycobacterium requires to carry out the specificity of the allergen on guinea pigs infected with a mixture of mycobacterium M. intracellularae and M. scrofulaceum and the guinea pigs infected M. bovis . The main point of the research consists of in the development of biological models and selection of dozes of Purified Allergen of atypical mycobacterium for the definition of size biological activity and specificity.

Work is devoted to research of consequences of influence of a sharp hypobaric hypoxia on reproductive cages of males of laboratory rats and mice. In experiment the rats of the HY/Y line, low-steady against a hypoxia brought and supported in SCBMT of FMBA of Russia and a mouse of the C57BL/6Y line supported also in our Center were used. Young sexually mature of males of rats and mice, having subjected to influence of a sharp hypobaric hypoxia, for 9-18 days of the post-hypoxemic period coupled to virginny females. For identification of consequences of acute hypoxemic stress on man's gametes, the accounting of embryonic mortality in the uterine period of development carried out, opening females for 19-20 days of pregnancy. It is established that at the general mortality of embryos in the post-implantation period of development in 32,3% in females of rats and in 40,2% at females of mice, the share of mortality of embryos caused by influence of a sharp hypoxia made 27,2% and 32,8%, respectively. Losses from among the ovulated ova at a preimplantation stage at females in experience made at rats - 16,6%, at mice - 5,2%. Thus, total losses under the influence of a sharp hypoxia on man's reproductive cages were equal at females of rats 43,8%, at females of mice - 38,0%. It is experimentally established also that the level of post-implantation embryonic mortality is in direct dependence on "quality of cleaning" (number of pairings) of a male on spermatozoa of the previous stages of a spermatogenesis. So, if after the 1st and 2nd pairing the size of post-implantation embryonic mortality made about 20%, after the 3rd and 4th - it increased to 46-78,8%.

The purpose of this study is the statistical analysis of experimental neurosis in laboratory animals. Analyzed the typical model of neurosis, showing their advantages and disadvantages. Dynamics of indicators of the emotional state of animals in the test elevated cross maze at different times of the formation of neurosis-like States was investigated. Quantitative assessment of neurotic animals takes into account both the ball an assessment of the performance of the test elevated cross maze and the severity of behavioral symptoms of neurosis. Developed a method of assessing the expression of the characteristics of the experimental neurosis in laboratory animals. It is established that for the formation of experimental neurosis in rats the best is spring and summer. Biomedical model of experimental neurosis can be used for preclinical studies investigating the effectiveness of new antiastheniс drugs.

Scientific interest in respect of detection of evolutionary regularities, and also practical interest in the field of medicine represents studying of influence of a xenotransplantation of stem cells on reproductive function of recipients. As alternative biomodels for experiment invertebrate hydrobionts - crawfishes as they are poikilothermic organisms with external fertilization were chosen, at them it is easier to track process of fertilization and development of germs and in ethical reasons. To sexual maturity crawfishes stem cells of marrow of donor mice in a dose of 8-10 million KKM were entered. It is revealed that introduction of stem cells to crawfishes initiates at them activation of reproductive function. Namely pairing, a caviar exit at females and its fertilization.

One of ways of restoration of parenchymatous bodies at violations of various genesis is transplantation stem cells. The purpose of the real researches was studying of regeneration of a liver thresh a carp with artificially caused pathology under the influence of stem cells of donor mice. Fatty dystrophy of a liver of a carp was caused by 7-fold introduction of per os paracetamol in a dose of 15 g/kg. Experiment showed survival of stem cells of mice of donors in a liver threshes a carp and successful regeneration of body. It was confirmed with detection of a gene of green protein and the polymerase chain reaction (PCR), and also detection of the donor fluorescing cages in cryocuts of a liver thresh a carp and a hepatopancreas of crawfishes. Thus hydrobionts have an intensive regeneration of fabrics and a complete recovery of the struck bodies. Results of researches will allow expanding possibilities of transplantation of stem cells in development of medicobiological researches of in the vivo models in the field of cellular technologies.