In article technologies of neuromodulation and its application in the context of psychoanalyses are consider.

On rats from the strain (HY/Y) low-steady against a hypoxia, deduced and supported in SCBMT of RAMS and on the mice strain C57BL/6Y supported also in collection fund of the Center, investigated consequences of influence of a sharp hypobaric hypoxia on viability of embryos. To acute hypoxia stress of females of both types subjected at the 10th bottom of pregnancy. As a result of the carried-out experiment it is established that the general embryonic mortality was equal in the postimplantation period of development in females of rats of skilled group of 9,5%, at females of mice – 11,2%. Thus the contribution recessive lethal in embryonic mortality at females of rats and at females of mice made 4,36 and 5,1%, respectively. Thus, the share of the lost embryos caused by influence of a hypoxia, equaled at females of rats of 6,0%, at females of mice – 6,1%.

To determine the processes of healing and osteointegration, after implant bed preparation with traditional burs in compare with piezosurgical tips were done on the 8 mini-pigs in experiment. The follow-up was 1, 30, 90 and 180 days. Radiological, histological, histomorphometric study were performed. In group with piezosurgical tips showed accelerating the integration and optimization of the wound healing processes.

It is hypothesized that impaired biomechanical properties of the sclera around the optic disc and, largely, in the corneoscleral shell may play an essential role in the pathogenesis of primary open-angle glaucoma (POAG). The paper’s objective is to study experimentally the impact of elastic scleral properties on intraocular fluid (IF) outflow. We determined the mechanical characteristics and the crosslinking level of the sclera as well as hydrodynamic parameters in three groups of experimental animals: 1) intact eyes of young (2 months of age) and old (2 years) rabbits; 2) eyes treated in vivo by threose, which increases crosslinking of scleral collagen; 3a) eyes of young and old rabbits after they were treated by collalysin, a proteolytic enzyme, 3b) eyes of rabbits pre-treated by treose and subsequently treated by collalysin. The hydrodynamic parameters were measured using Glautest 60. Differential scanning calorimetry was used to reveal the crosslinking level of scleral collagen. The biomechanical parameters of sclera were determined by Autograph AGS-H. The normal age-related increase of scleral stiffness (Young’s modulus) from 23.1±4.2 MPa to 41.4±6.3 MPa (p<0.05) is followed by a moderate growth of cross links and a somewhat reduced IF outflow. At the same time, an increase of scleral stiffness (to 65.4±6.0 MPa) caused by threose, i.e. a pathological growth of crosslinking, is accompanied by a certain intraocular pressure increase and a significant impairment of IF outflow. Collalysin- treated old rabbits revealed a fall in scleral stiffness (to 27.9±4.9 MPa) and an improved IF hydrodynamics, whereas young animals showed only a slight change in these parameters. The sclera treated by threose and containing excessive cross links also became less stiff (43.4±4.5 MPa) and improved IF hydrodynamics after collalysin treatment. It may be assumed that the biomechanical properties of sclera affect the eye hydrodynamics: IF outflow is deteriorating with the increase of scleral stiffness that is caused by excessively generated cross links in its collagen structures, which may be a risk factor for POAG. Proteolytic therapy with collalysin helps reduce the amount of these links, make the sclera less stiff and improve hydrodynamic parameters of the eye.

Isotope effects of deuterium (D) in cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophical, chemoheterotrophical, photoorganotrophical, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria, halobacteria, green microalgae) are investigated at growth on media with heavy water (D2О). The method of step by step adaptation technique of cells to D2О is developed, consisting in plating of ctlls on 2% agarose nutrient media containing increasing gradient of concentration of D2О (from 0 up to 98% D2О) and the subsequent selection of stable to D2О cells. Obtained from growth media with a low gradient of D2О concentration, cells were further transferred on growth media with higher gradient of D2О concentration, up to 98% D2О. In the result of that technique it were obtained adapted to maximum concentration of D2О cells, biological material of which instead of hydrogen contained deuterium with levels of enrichment 95% D. Usage of cells adapted to D2О and deuterated natural compounds (protein, amino acids, nucleosides, pigments, carboxydrates, lipids), extracted from them, can find further application in biophysical researches and biomedical technologies

Comminuted leg fractures were modeled in 18 adult mongrel dogs. A complex histological study was performed using computer morphometry of the femoral and anterior tibial arteries, the superficial peroneal nerve, the knee cartilage in different periods of leg fixation by the Ilizarov device (days 14, 21, 35-37 and 48-50), 30 and 90 days after the device removal (80 and 140 days of experiment, respectively). The signs of the anterior tibial artery mechanical injury have been revealed in 22,2% of experiments, those of the peroneal nerve injuries – in 11,1% of cases. The dynamics of changes in the medium diameter of the anterior tibial artery of the limb operated, lumen diameter and its wall thickness has been established to depend on osteogenesis intensity. The increase of the absolute value of morphometric parameters has been noted in animals with accelerated fracture consolidation (35-37 days), in case of the device fixation for 48-50 days all the morphometric parameters have had the tendency towards increasing in comparison with fixation for the period of 14-21 days. The increase of arterial calibre and lumen was accompanied by the formation of the defects of the inner elastic membrane the length of which in cross sections exceeded 30% of luminal surface perimeter. In the experiments with the absence of the signs of peroneal nerve mechanical injury the changes in epineurium and perineurium have been revealed evidencing the intensification of the structural-and-functional characteristics of the perineural barrier, as well as histogenesis of the structures providing the nerve mechanic strength and the maintenance of homeostasis of nerve fiber microenvironment medium. The destructive changes have been revealed in the articular cartilage expressed as follows: the visualization of the superficial zone extracellular substance garnetting, the death of a part of chondrocytes, the decrease of cartilage thickness and chondrocyte numerical density. The reparative regeneration of the cartilage was of incomplete for up to 140 days of the experiment. The experimental data obtained are required for developing the effective methods of supporting therapy and functional rehabilitation of casualties with bone fractures.

The iпfluence of emoxipine on the structure of the thyroid gland of the young and old white rats under immobilization stress is investigated. The study found that emoxipine eliminated changes in the structure of the thyroid gland the height of the thyroid epithelial cells and the area of the nucleus were increased, the area of the follicles and the area of the follicular colloid were decrease.

Lines and families of existing population of Svetlogorsk mini-pigs their main characteristics and features are described

The pharmacokinetics of lipid-lowering drug of the statin – Аtorvastatin by HPLC In 18 healthy humans are examined. In 30 patients with hyperlipidemia studied the clinical efficacy of Atorvastatin. The treatment marked by a pronounced gipolipidnemic effect with a significant decrease in atherogenic lipides profile. A slight antiplatelet effect of this drug also noted.

Article presents results of the study of new bandaging material's direct contact and indirect cytotoxicity assays in comparison with cotton gauze. It have been found that in direct contact of nonwoven polymeric fibrous bandaging material (BM) with cells for 24 hours of cultivation no changes in cell morphology take place, nor does the amount of dead cells increase. These conclusions have been made by means of both a visual examination and an МТТ assay. The BM extract did not have any cytotoxic effect on the tested cells either. The obtained results allow us to make a conclusion that the BM complies with the international standard ISO 10993-5, which is applied to medical goods, and can be applied in the treatment of infected wounds in clinical practice.

Paper is devoted to the application of epithelial monolayer of human colon adenocarcinoma cells – Caco-2 for in vitro permeability evaluation of drugs. Main advantages of this biological model are described. General principles, approaches and methodology of Caco-2 permeability assay are defined. Correlation between human intestinal permeability in vitro and apparent permeability coefficient in vitro is indicated.

Despite of wide arsenal of drugs for narcosis, existing now, quite often the anaesthesiologist is faced with the problem of optimum diagram of anaesthesia. Evaluation age, social, emotional characteristics of patient is often not enough. That is why studying SNP-polymorphisms and their influence on a metabolism of anaesthetics, probably will allow to optimize the concept of «individual approach» in anaesthesiologia. A substantial part of the individual variability of person response to drugs is related with single nucleotides polymorphisms. Drugs that are used in general anesthesia undergo polymorphic metabolism. Among these, CYP2B6, CYP3A4 and CYP2C9 play a relevant role in the metabolism of ketamine. The enzymes involved in the metabolism of thiopental are still unknown. Propofol is metabolized mainly by glucuronidation by uridine diphosphate-glucuronosyltransferases (UGTs) and by hydroxylation by CYP2B6 and CYP2C enzymes. The enzymes SULT1A1 and NQO1 participate in later steps in propofol metabolism. CYP3A4, CYP3A5, CYP2B6, CYP2C19 play significant role in later steps in propofol metabolism. CYP3A4, CYP3A5, CYP2B6, CYP2C19 play significant role in the metabolism of benzodiazepines. The present review analyses the importance of enzymes in the metabolism of anaesthetics.

The results of experimental modeling canine osteoarthrosis by the knee immobilization with the femoral artery transsection are presented in the work. The results of the assessment of the articular cartilage morphofunctional state evidence the development of degenerative-and-dystrophic changes manifested as the death and destruction of a part of chondrocytes, the disorder of extracellular matrix homogeneity and the decrease of the parameters evaluated by morphometry – cartilage thickness, volumetric and numerical density of chondrocytes. The changes marked appear to be a consequence of metabolic process disorders in the articular cartilage evidencing the decrease of synthesis and the predominance of catabolic processes. Under these experimental conditions the intermediate zone chondrocytes equidistant from the vascular and synovial supply factors turned out to be the most vulnerable ones.

The positive therapeutic effect of the cultivated cells of a marrow on correction of pathogenetic violations of periodontosis at genetic mice of the BRSUNT line is studied.

In the present publication the method of identification neuroleptics – derivatives benzamide (amisulprid, sulpirid and tiaprid) in extraction from blood is offered. The offered technique allows with high reproducibility and reliability to carry out identification of studied substances with so-extractive substances.