The study examined the role of acetylation in phenotypic and epigenetic mechanisms of gene expression that do not affect the original sequence by the action of procancerogenic and antineoplastic drugs. Based on the data obtained in vitro experiment on enzymatic activity, gene expression and epigenetic NAT2hom factor of action with an effective inhibitory effect bisphenol substances (nitrosobenzene and diacetoxybiphenyl) and cisplatin for the enzyme N-acetyltransferase NAT2 human. For selected inhibitors studied concentration dependence of the enzymatic activity and determined the concentration value premaxillae inhibition IC₅₀. Create a new model of in vitro evaluation acetylation ability as well as determination of the kinetic parameters of the acetylation of substrates, activators and inhibitors, NAT. The model proposed for the screening of substances that affect the process of acetylation. With its help, as well as on models of transgenic mice carrying the NAT2 gene of the person and the axolotl the nature of the interaction in the "inhibitor - enzyme activity", "inhibitor of expression of a gene" and the predicted level of toxic effects of NAT inhibitors on enzymatic, genetic and epigenetic levels. Experimentally on the example of the impact of the molecules of the toxicants presents a model study of epigenetic factors derived bisphenol and cisplatin in the regulation of epigenetic programs violations regeneration remote extremities of the axolotl, which is expressed in slower growth and changes in the formation of the fingers. There was deceleration of erythropoiesis and increased blood. Cisplatin and derivatives bisphenol cause an increase in the proportion of eosinophils and content of lysosomal cationic protein in neutrophils and a significant increase in the percentage of monocytes, which as a whole confirms the development of oncological processes of any localization.

Development of the blood-brain barrier model in vitro is one of the main points in modern Neurobiology and Neuropharmacology. Application of microfluidic approach to the blood-brain barrier modeling allows studying and reconstructing the key events in cerebral angiogenesis, barriergenesis, regulation of structural and functional integrity of the barrier, and testing the drug candidates targeting brain tissue. Here we review the current achievements in the development of blood-brain barrier microfluidic models as well as the most promising technological solutions to get novel opportunities in the development and application of these models in vitro .

Developed modification method repetitious swimming with loading 10% of body weight, which allows more clearly assess the endurance and speed the formation of fatigue in rats. A statistical analysis of the methodology allows producing a population transfer of the results obtained. On the basis of an analysis of the breakdown of points of efficiency in forced swimming with loading test 10% of body weight and kinezohydrodinamic studies offered new aspects of the interpretation of the data.

We identified new and confirmed the previously known types of biological activity for some of the most common cyanobacterial metabolites (microcystins, anabaenopeptinov, saxitoxin) by computer simulation. Within the design based on the target structure (structure-based drug design) was studied docking interaction (3D-QSAR) involving cyano-metabolites molecules. Based on the results of the study the most promising candidates were selected for further study - neosaksitoksin (neosaxitoxin), goniatoksin II (gonyautoxin GTX2) and aeruginozid 126A (aeruginoside 126A), for which the observed properties of the manifestation of cytostatic, anti-tumor antibiotics and anticoagulants.

The brain sexual dimorphism indicates the morphological differences between individuals. Amygdala complex (MK) of the brain is one of the areas of sexual dimorphism. Bilateral relations of the MK with the hypothalamus ensure its participation in the processes of puberty and the regulation of the gonads, that allows considering this area as a link in the reproductive system of the organism is located on its territory neuroendocrine centers, which is able to regulate the secretion of gonadotropins and sexual behavior. On the territory of the anterior part of the MK zone of sexual dimorphism presented by the front amygdalar area (AAA), front cortical (COA) and Central (CE) nuclei, having the ability to regulate the secretion of gonadotropins and sexual behavior.

Natural ligands protono-aсtivated receptors were studied by biomodel (hypersensitivity provoked by CFA and visceral pain) assessment of somatic pain. Peptide UGTX study pharmacological activity after intramuscular hypersensitivity tests provoked CFA, and visceral pain, showed the maximum analgesic activity at a dose of 0.02 mg / kg. Increasing or decreasing the dose of UGTX peptide was not significantly different from the reference drug. Sevanol 1 mg / kg showed comparable diclofenac analgesic activity, but at a dose of 10 mg / kg of diclofenac significantly superior in terms of reduction of the total number of cramps. The studied ligands may be considered as potential analgesic drugs.

Pharmacoresistance to antiepileptic drugs (AEDs) is one of the major problems in the treatment of epilepsy. According to one of the current hypotheses, this pharmacoresistance may be associated with the reverse transport of AEDs by drug efflux transporters such as P-glycoprotein (Pgp) through the blood-brain barrier. However, so far there is no unequivocal opinion regarding the involvement of P-gp in the resistance to carbamazepine, one of the most important AEDs. The objective of the present study was to investigate the influence of verapamil, which is known to be P-gp inhibitor, on the anticonvulsive effect of carbamazepine in rat epilepsy induced by isoniazid. A significant enhancement of the anticonvulsive effect of carbamazepine in the presence of verapamil (30% decrease of the effective dose - from 30 mg/kg to 20 mg/kg) suggests that P-glycoprotein may considerably limit the transport of carbamazepine to the brain.

The work is devoted to the research of Epadol influence on the structure of liver in animals with experimental hypothyroidism. Over 50 animals were examined. Cells of liver were described with particular attention to the structure of their organelles. It has been established that consumption of Epadol a pronounced effect on the ultrastructure of the cells of the liver.

The effect of hypochlorhydria on qualitative and quantitative composition of the luminal microflora in the jejunum and cecum of rats was studied. It is shown that intragastric administration of pariet for 16 days leads to the Escherichia coli colonization of the proximal small intestine, which is accompanied by loss of body weight. It is found a decrease in the composition of microbiota in the jejunum and cecum. Qantitative disappearance of the proximal-distal gradient of microorganisms in the intestine was detected.