Methodical features of the formation and study of acute general hypothermia in laboratory animals - white rats are considered. It was revealed that the initial animal population is not homogeneous by the level of resistance of animals to hypothermia, and "resistant" and low resistant to animals are distinguished in it. Indicators reflecting the dynamics of the thermal state of animals (the rate of heat accumulation or heat loss), by the features of their statistical distribution are far from normal and cannot be used for population transfer and interpretation of the data. The indices, based on the performance characteristics of animals under immersion cold exposure, more correctly reflect the dynamics of compensatory-adaptive processes and may be considered preferable in studies on the specific frigoprotective activity of new drugs. In studies using reference psychomotor stimulators (sydnokarb, caffeine), the specificity of the developed experimental model was confirmed.

Convergent validation of intracentral relations is based on the generality of the results obtained with the help of various methods and criteria for evaluating the work of the brain and higher nervous activity of the studied animal biomodels. A software-hardware complex (neuromodule) is developed, which is necessary for studying the psychophysiological, electrophysiological and psychopharmacological aspects of intracentral brain relations. The methods and approaches laid down in the structure of the neuromodule are described in the article. In the chronic experiment, the make electrode constructions were implanted into informative regions of the brain of cats. By means of local electrical activation of the brain, the evoked potentials and the parameters of the electroencephalography were recorded, for the interpretation of the results of which software was developed on the basis of modern mathematical techniques. A unified database and a data bank are compiled for all animals that are studied with the help of these techniques, which facilitate the objectification of the results of the analysis of intracentral relationships of the brain.

The main symptom of Alzheimer's disease is rapidly progressive dementia, which correlates with the formation of amyloid plaques and neurofibrillary proteins in tissue of the brain. This leads to the death of neurons, synoptic and cognitive dysfunction. The aim of the study was to study the development of social and cognitive impairments in the process of ontogenesis in animals with a genetic model of Alzheimer's disease. The object of the study is a genetic model of Alzheimer's disease is the B6SLJ-Tg line mice (APPSwFlLon, PSEN1 * M146L * L286V) 6799Vas, males aged 4 months. Control group - C57BL / 6 x SJL mice, males aged 4 months. Neurobehavioral testing included the following protocols: test «open field», elevated plus maze, light/dark transition test, a three-chamber social test, social recognition memory test in rodents, test «Fear conditioning». We identified features of behavioral in animals Tg 6799 line with the development of Alzheimer's disease. This increase the level of anxiety and lethargy, impaired social contacts and interactions, as well as spatial, social and associative learning and memory. Together, this indicates a disorder of emotional behavior, as well as long-term, social, spatial and emotional memory.

This study reports a simple¹⁴C-breath test in rats. The expired carbon dioxide in the chamber was collected in a breath-sampling system using a pump after intravenous injection of¹⁴C-glucose. The level of¹⁴CO₂ in the expired air was measured at 10-min intervals for 30 min. The received data confirm that this method is suitable for medical and biological investigations using labeled compounds.

In order to more accurately assess the safety of research in pre-clinical studies have proposed the technique of an estimation of system of detoxification of xenobiotics in laboratory animals. The study was performed on two species of laboratory animals - frogs and rats. As toxic xenobiotic used two medications with fundamentally different mechanisms of action and different ways of detoxification: ethyl alcohol and cisplatin. Scoring of toxic action of xenobiotics was carried out by the method of expert scaling. Thus, the study showed that the effectiveness of the detoxification system of xenobiotics can be judged according to their Toxicological studies acute toxicity on the basis of the dynamics of the total point scoring of severity of signs of intoxication.

The article presents the results of a study of the solution of the sodium salt of chitosan succinamides (5 %) effect and dispersions of the sodium salt of chitosan succinamide c AgJ (1:1) on the activation processes of immunocompetent cells in vitro. It is shown that the solutions of the sodium salt of chitosan succinamide and dispersion of colloidal particles of silver iodide increase the phagocytic and metabolic characteristics of neutrophils, cause a moderate increase in expression of activation receptors CD25+, СD95+ and HLA-DR+ lymphocytes, and increase the proliferative activity of lymphocytes along with an increase in the number of cells with intracellular apoptosis signs.

Zootechnical assesment conducted in five breeding lines of Svetlogorsk mini-pigs. Characteristics of animals identified in each line and selection work with livestock planned. Obtained animals with reduced body weight, of the desired type in each line that meet the necessary requirements for laboratory animals. Culled animals Vs. line, which resulted in the need to create new lines. As well as increase of multiple pregnancy in lines. We plan to study other indicators for each line (performance, behavior, etc.).

The influence of a novel diethylaminoethanol derivative (10 and 75 mg/kg) was studied in the following tests: conditional reaction of passive avoidance, «Extrapolation deliverance» and «T-maze» tests. FDES had a protective impact on the consolidation and reproduction of memory trace in 2 and 24 hours after scopolamine was administered, and its effect was comparable to that of piracetam. Furthermore, the novel diethylaminoethanol derivative facilitated the retention of the ability to choose the "correct" arm in the T-maze test and also outperformed the reference drug (agent). During the administration of FDES in both doses, in the EP test, the rats dove under the edge of the cylinder earlier, also the number of rats which dove was higher in comparison with rats to which piracetam was administered.