The effect of the release product of the musk glands of Moschus moschiferus was determined by determining the expression levels of the molecules of the 1α subunit of the hypoxia inducible factor, inducible nitric oxide synthase and cyclic guanosine monophosphate -specific phosphodiesterase type 5A in experimental animals under stress caused by forced activity. The biological materials (brain, heart and lungs) of Wistar rats that received the substance under study and undergone a forced swim test with a cargo were examined in the work. The gene expression profile was determined by droplet digital polymerase chain reaction. Against the background of the introduction of test substances, the expression levels of the studied are characterized by the expected values, which demonstrate the accelerated start of multifactorial mechanisms of adaptation of the organism and amplifies the ability of tissues to experience unfavorable conditions upon administration of active components of the musk glands of Moschus moschiferus .

This review relates to insulin signaling system in the brain, which differs from those in peripheral tissues. Brain cells express predominantly, and neurons exclusively, the high affinity short isoform A of the insulin receptor (IR-A). IR-A binds insulin and insulin-like growth factor 2 (IGF-2) with physiologically relevant affinity, in contrast to isoform B (IR-B), major isoform in adult peripheral tissues. IR-A is involved in the regulation of synaptic plasticity, the growth of dendrites and axons, and the regulation of adult neurogenesis. The insulin system in the brain plays a role in the processes of memory formation and the regulation of eating behavior. Impairments of insulin signaling in the brain lead to the development of central insulin resistance, the most prominent manifestation of which is Alzheimer's disease. The development of drugs specifically targeting central insulin resistance represents a new promising approach in the treatment of diseases of the central nervous system.

Of particular interest in the field of synthesis are derivatives of N-substituted antranilic acid (AA), due to their versatility of biological effects and low toxicity. Structural modification of AA derivatives affects the expansion of the spectrum of pharmacological action; therefore, they have been used and are used in medical practice as antiinflammatory, analgesic, antipyretic and diuretic medicines. The aim of this work is a computer prediction using a computer program PASS Online, synthesis and experimental study of biological activity of new compounds, some substituted hydrazides of NH-acyl-5- iodantranylic acid. The synthesis of new compounds was carried out using methods of cyclization, amideation, condensation. The purity of the obtained products was controlled by TLC on plates Sorbfil PTSH-P-V. The structure of compounds was established by IR spectrometry and¹H NMR spectroscopy. Antibacterial and antiinflammatory activities were studied in the laboratory. Thus, the obtained data on the prediction of the biological effect and the tests carried out testify to the prospect of searching for pharmacologically active compounds in the series of NH-benzoyl-5-iodoanthranilic acid R-benzylidenhydrazides.

The aim of the study was to study the antagonistic activity of strains of Lactobacillus fermentum BKM B-2793D, Lactobacillus reuteri BKM B-3144D isolated from human breast milk and their consortium to pathogens that cause mastitis during the lactation period. The lactobacilli and their consortium reveal antimicrobial activity against the indicator microorganisms used in this study including pathogens resistant to antibiotics. The consortium protects human mammary gland MCF-7 cells against adhesion and colonization by Staphylococcus aureus 8325-4. The obtained results suggest that lactobacilli from breast milk of healthy woman L. fermentum ВКМ В-2793D and L. reuteri ВКМ В-3144D and their consortium are excellent candidates for the development of probiotic preparation for prophylactics and treatment of lactational mastitis.

Due to the widespread use of tobacco and accessories for it, a lot of teenagers in the world and in our country inhibit the gas to refuel lighters (butane), this phenomenon is called "sniffing". Under the influence of butane there are cases of sudden death, often associated with physical exertion. Butane is an inhalant drug that sensitizes the myocardium to the arrhythmogenic effects of catecholamines. In experiments on rats, an arrhythmogenic and secondary ischemic effect was shown with inhalation of butane.

The purpose of this work is to compare of the morphofunctional characteristics of the lower leg muscles in conditions of transosseous distraction osteosynthesis by high-rate and high-fractional automated destraction and in combination with intraosseous reinforcement with a titanium. The main experiments were performed on mongrel dogs of both sexes at the age of skeletal maturity, lengthening of the tibia was performed with a daily autodistractor at a rate of 3.0 mm for 10 days. In the animals of group No. 1, the autodistractor step was 0.025 mm at 120 times. At the general anesthesia, intramedullary reinforcement of the tibia was a 1.8- mm titanium (Ti6Al 4V) coated with hydroxyapatite by microarc oxidation technology. Next, osteosynthesis with an Ilizarov apparatus and transverse osteotomy of the tibia were produced. In the group No. 2, the autodistractor step was 0.017 mm at 180 times, тhe fixation period was 30 days. At the end of the distraction and 3 months after the removal of the apparatus, the tibial muscles were examined. Animals of the group No. 1 actively used the limb from the first days of distraction. The period of fixation decreased twice. Macro-preparations of muscles of dogs in group No. 1 did not significantly differ in the experimental and contralateral limbs. The histostructure of the muscles in both groups was characterized by signs of activation of angiogenesis and myogistogenesis, but in the group No. 1 the volume density of microvessels was 3 times higher in the period of elongation. It was concluded that the method of high-speed limb auto-extrusion in combination with intramedullary reinforcement of titanium wire with hydroxyapatite spraying has an activating effect on angiogenesis and myogenesis. The histostructural characteristics of the tibial muscle testify to the predominance of the processes of reparative regeneration by the type of restitution. As a result, the functional activity of the limb remains from the first days of distraction, the period of fixation is reduced by 2 times.

There is data present in this research on the effect of nutrition products prepared using cryogenic technology on oxidative stress, serum antioxidant capacity, glutathione levels and metabolites of nitric oxide in swimmers during intensive physical loads. It was demonstrated that the efficiency of antioxidant systems, glutathione systems and nitric oxide relative increased in athletes who took the food product in comparison to those who took a placebo.

Comparison of the gestagenic activity of synthetic analogues of progesterone - ethers of acetomepregenol (AMOL) determined in the process of studying by the various biological tests, showed the absence of correlation between the results obtained in vitro and in vivo . At the low affinity to the progesterone receptor AMOL ethers exhibited the high progestational activity in animals that are likely to be explained by the occurrence of receptor-substrate interaction after hydrolysis of the ester bond by the action of esterases in organism. Established in vitro models, the absence in the AMOL ethers of undesirable glucocorticoid, androgenic or antiandrogenic effects, along with their high in vivo gestagenic activity, makes them promising for use in medicine and veterinary medicine.

The preconditioning effect of different pulsed magnetic field regimes on hematological and biochemical parameters of blood of rats exposed to hypobaric hypoxia was studied. It was established that the impact of pulsed magnetic field leads to the development of adaptive response, however, the rate of its activation can be different depending on the selected mode. The possibility of using a low-frequency pulsed magnetic field as a protector of a stress-induced condition caused by hypoxic exposure is shown.

The influence of the new aminoethanol and dicarboxylic acids derivative (FDES) on endurance, swimming speed, morphological and biochemical changes in the animals during aerobic training loads was evaluated in comparison to the synthetic adaptogen with strong actoprotective action - ethylthiobenzimidalozol hydrochloride. As a result, it was found that an increase in physical performance in the application of FDES is comparable to that in the application of ethylthiobenzimidalozol hydrochloride. Animals endurance, estimated by the number of passed pools and an increase in the total swimming time, in group, which received FDES, increased by 1.92 and 3.14 times, respectively. Changes in swimming speed were not noticed in both groups. The application of FDES during training process contributes to the development of compensatory hypertrophy of myocardium.

Immunohistochemical method using mouse polyclonal antibodies was used to study the expression of Ki-67 antigen cell proliferation markers and phagocytes-1 growth factor (FGF-1) in mature female rats (12-13 months) with intradermal mesotherapy of unmodified bovine collagen type I, as an analogue of which we used the domestic preparation "Collost® gel". It has been established that unmodified collagen stimulates the proliferation of skin epithelial cells and hair follicles, fibroblasts, and vascular endothelium. The most pronounced expression of the cytokines studied is observed on the 7-21th day after the injection of the collagen preparation.