Лекарства, проникая в организм, изменяют его потенциальную энергию, а через некоторое время и кинетическую, что выражается в поведенческих реакциях животных и человека. Это означает, что энергия является наиболее важным параметром на всех стадяих создания лекарств – от молекулярной биологии до клинических исследований. Система Laboras, созданная компанией Metris B.V., измеряет общую кинетическую энергию, в отличие от других автоматизированных систем, распознающих поведение только по энергии передвижения животных. В результате использования системы Laboras исследователь получает такие формы поведения животных как встряхивание головы, подергивание головой, «отряхивание мокрой собаки» и почесывание, не распознаваемые другими системами. Система Laboras может интегрироваться с DSIтелеметрической системой и, таким образом, анализировать физиологические и поведенческие параметры одновременно. Система также интегрируется с системой Sonotrack для изучения ультразвуковой вокализации.

In this article have been discussing the algorithm of neuromodulation methods and creation toolmaking counterpart of the transactional analysis having used for ego-states alterations and the correction of individual script.

We tested action of oxygen, ozone (500 mcg/l), nitric oxide (800 mcg/l) and singlet oxygen on human blood specimens in vitro. Estimated parameters were lactate dehydrogenase activity, blood lactate and some special coefficients. It was stated, that blood oxygenation, ozonation or processing by singlet oxygen stimulated energy reserves, and nitroxylation depressed it.

Comparison of the efficiency of the laser ablation of biological tissue with the use of a repetitively pulsed CO2-laser with transverse discharge and a continuous CO2-laser with modulated output radiation is carried out. It is shown that the ablation efficiency is higher for the repetitively pulsed laser with a pulse duration of 20 .s and a pulse power of more than 2 kW, and the radiation from the cw laser with the power of 50 W and pulse duration of 500 .s results in much greater thermal damage to the tissue surrounding the impact zone. The first clinical application of the repetitively pulsed CO2-laser in dermatology and plastic reconstructive and restorative surgery has shown its clinical and aesthetic performance and identified key long-term direction of its further use for surgery.

The action of organophosphorus compounds (OPC) O-ethyl-S-geksilmetiltiofosfanata on the activity of serum cholinesterase and factors of nonspecific resistance purebred mice has been studied. LD50 of O-ethyl-Sgeksilmetiltiofosfanata for neinbrednyh mice after intraperitoneal injection of 12,33 ± 4,05 mg / kg. Inhibition of serum cholinesterase remains within 24 h after injection. Organophosphorus compound has a pronounced immunotoxic effect on the factors of innate immunity, causing leukopenia, inhibition of functional activity of neutrophils and macrophages (decrease adhesive, phagocytic and bactericidal activity).

Experiments on white Wistar rats the effects of stress on the effects of diazepam (0,1 and 0,5 mg/kg) in intact and adrenalectomized male and female rats examined with multi-parameter testing at 6, 9, 12, 15, 18, 21, 24, 3 o'clock. After stressing sedative effect of diazepam in intact males weakens, displacing the early morning hours and on the contrary in the female increases, inducing in the evening. In addition, stress the activating effect of the drug in females and anxiogenic effects in male and female rat’s levels. Also, stressing significantly antianxiety activity of diazepam in intact males weakens, and in females dismissed by the evening time in the morning. Adrenalectomy sedative and antianxiety attenuates it is shown, anxiogenic effects of diazepam in males negates. In females, the adrenal gland removal the sedative and activating effects eliminates, antianxiety activity shifts in the early evening time and the anxiogenic effects of tranquilizers in a dose of 0, 1 mg/kg induces and of 0, 5 mg/kg levels. Stress on the background of adrenalectomy males sedative effect of low dose eliminates and high dose of diazepam induces, significantly antianxiety weakens and the anxiogenic effects of the drug induces. Sedative and antianxiety effects in adrenalectomized females after stressing reinforce, anxiogenic effects of tranquilizers eliminate.

This review covers basic fundamental models of experimental modeling of depression and methods of experimental modeling of behavioral alterations. Animal models are promising enough for the understanding of psychopathotology, but not as a deviations of behavior, as well as a series of psychological processes that obey certain laws. Creating models of depression is experiencing a period of prosperity. There are several models of depression: chronic mild stress model, social conflict model, leaned helplessness model, water- immersion model. Many of them are based on some manipulation of the animals behavior, which have know biological effects. The investigation of the behavior of laboratory animals and modeling of depression allows detail characteristic features of psychopatology in humans.

Morphometric characters of laboratory animals measured in different laboratories by different investigators are varied essentially. Apparently, this difference is caused by various influences of environment, depends on the way, moment and a place of blood sampling, dietary, animal age and some other factors. Standard conditions of animal’s maintenance are necessary for valid and reproducible research data therefore much attention now is given to the experimental animals care and use. This paper summarizes morphometric characters of SD (Sprague Dawley) rats mice which had SPF status (specific pathogen free) and were housed in standard conditions recommended for laboratory rodents maintenance.

The conducted research showed that mice of various genotypes possess different resistance to a sharp hypobaric hypoxia. On sensitivity to a sharp hypoxia studied genotypes can be divided into 3 categories conditionally: the low-steady – IOR/HabY, CC57WY, CBA/LacY, 101/HY; middle-steady – C3HAY, CBA/ JY and high-steady – A/SnY, B10CWY, C57BL/6Y, DBA/2Y. Distinction between highly and low-steady against a hypoxia are statistically reliable (p> 0,001). It is necessary to specify that inbred age thus has no impact on this sign. That fact testifies to it that the mice of the most highly inbred CBA/JY line who have passed 251 inbred generations, reached average position between low and high-steady genotypes. According to the received results, the studied sign possesses high variability both between animals of different lines, and between individuals in each line. High variability in sensitivity to a sharp hypoxia at animals of one genotype testifies to lack of selection on this sign. Distinctions between females and males in lines are doubtful. The exception was made by mice of the CBA/LacY line at whom males to a sharp hypoxia authentically surpassed females in sensitivity.

The paper evaluated and analyzed the effect of drugs that affect the body’s ability to withstand the limit physical activity (eg, resistance to acute hypoxia) and, therefore, increase endurance and performance. Preclinical testing antihypoxic activity of new chemical compounds - animal peptides were performed on rats of Wistar. Found that all study drugs resulted in high resistance of experimental animals to acute hypobaric hypoxia (significant prolongation of life on the “height”) -is 7-8 times higher than the background rate. Antihypoxic greatest activity at 21 days following initiation of administration have shown in the first part of the experimental drugs under the code Pep-6, Pep-5 and Pep-4, in the second - Pep-12, Pep-8 and Pep-11. The results of biochemical and hematological studies indicate no adverse effects of extreme physical stress on the body of rats and an overall positive effect of study drug. Antihypoxic effect is accompanied by a significant increase in horizontal motor activity and changes in the mechanism of blood lactate. Thus, the investigated drugs are antihypoxants have the property to increase endurance and stamina, experiencing physical stress. In this connection, they can be recommended as a means of stimulating action when working in extreme situations and under conditions of high physical activity, including sports pharmacology.

In article summariezes the material experimental studies on the early response gene с-fos in the cells of the nervous system and protein с-fos, which it initiates synthesis of variety experimental conditions. It is shown, in particular, the involvment of gene and its products in the physiological and pathological processes proceeding in the individual structures of nervous system.

Individual variation in the response to drug therapy has been mainly attributed to the genetic polymorphism of cytochrome P 450 isoenzymes. We examined 40 patients with CHD, who received clopidogrel and 146 healthy volunteers. CYP2C19*2, CYP2C19*3, CYP2C19*17 carriage was determined by a polymerase chain reaction. 20 clopidogrel-treated patients had the CYP2C19 *1/*1 genotype (50%), 12 – CYP2C19 *1/*2 (30,0%), 5 – CYP2C19 *1/*17 (12,5%), and 3 – CYP2C19 *17/*17 (7,5%). Among the healthy volunteers, 111-CYP2C19 *1/*1 (76,1%), 31 – CYP2C19 *1/*2 (21, 2%), and 4 – CYP2C19*2/*2 (2,7%). The frequencies of CYP2C19*2 were 15,0% and 13,3% in patients with CHD and in healthy volunteers respectively (p=0,323). The results of the present study show the impact of individual response variability to clopidogrel on clinical outcomes and may be helpful in developing current and future directions for its management.

In current clinical practice, there has been significant growth in the use of antibacterial drugs for the treatment of patients with a variety of infections. Beta-lactam antibiotics are among the most clinically important antibiotics used in medicine. Despite its high efficacy in the treatment of many infections, the scope of the beta-lactam antibiotics significantly limited adverse reactions that occur during treatment with these drugs. The most common of them in clinical practice is an allergic reaction. Beta-lactam antibiotics are drugs that are most often develop IgE-mediated allergic reactions. Now for the prediction of such side effects, numerous studies to find candidate genes involved in the development of allergic reactions to betalactam antibiotics. This article looks at the frequency of adverse reactions and structure of allergic genesis to beta-lactam antibiotics, the pathogenesis of allergic reactions, as well as strategies to find potential genetic predictors.